About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:189472 Armendariz BG, et al., Expression of Semaphorin 4F in neurons and brain oligodendrocytes and the regulation of oligodendrocyte precursor migration in the optic nerve. Mol Cell Neurosci. 2012 Jan;49(1):54-67
Assay type: RNA in situ
MGI Accession ID: MGI:7860842
Gene symbol: Sema4f
Gene name: sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain
Probe: Sema4f probe
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 14.0 (more )
Structure Level Pattern Image Note
TS22: telencephalon Present Widespread 1A Widely expressed in most brain regions during development.
TS22: cortical plate Very strong Regionally restricted 1A Expression in young postmitotic neurons in cortical plate.
TS22: ganglionic eminence Strong 1A Expression particularly high in proliferative layers - e.g. ganglionic eminence at E14.
Specimen 1B: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: telencephalon Strong 1B Expression particularly strong at E16-P0.
TS26: hippocampus Very strong 1B Expression in cells resembling glial cells. Expression in oligodendroglial precursor migratory pathway of hippocampus. Expression in embryonic fimbria.
Specimen 1C: postnatal adult (more )
Structure Level Pattern Image Note
TS28: forebrain Present 1C Expression still significant in adult, although clearly decreased.
Specimen 1D: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: telencephalon Strong Regionally restricted 1D Expression particularly strong at E16-P0. Expression particularly high in proliferative layers - e.g. neuroepithelium at E16.
TS24: cortical plate Very strong Regionally restricted 1D Expression in young postmitotic neurons in cortical plate.
Specimen 1E: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: telencephalon Strong 1E Expression particularly strong at E16-P0.
TS26: hippocampus Very strong Regionally restricted 1E Expression in early pyramidal and granule cells, cells resembling glial cells and oligodendroglial precursors in migratory pathway of hippocampus. Expression in embryonic fimbria
Specimen 1F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: hippocampus Present 1F Expression still significant in adult, although clearly decreased.
Specimen 1G: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: telencephalon Strong 1G Expression particularly strong at E16-P0.
TS26: corpus callosum Present Regionally restricted 1G Expression in cells of oligodendroglial shape as early as P0.
TS26: telencephalon septum Present 1G
Specimen 1H: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: forebrain Strong 1H Expression particularly strong at E16-P0.
TS27: telencephalon subventricular zone Strong Regionally restricted 1H Expression particularly high in proliferative layers - e.g. anterior subventricular zone at early postnatal stages.
Specimen 1I: postnatal adult (more )
Structure Level Pattern Image Note
TS28: telencephalon Present Regionally restricted 1I Expression still significant in adult, although clearly decreased.
TS28: entorhinal cortex Present 1I
Specimen 1J: postnatal adult (more )
Structure Level Pattern Image Note
TS28: telencephalon Present Regionally restricted 1J Expression still significant in adult, although clearly decreased.
TS28: rostral migratory stream Present 1J
Specimen 1K: postnatal adult (more )
Structure Level Pattern Image Note
TS28: main olfactory bulb Present Regionally restricted 1K Expression still significant in adult, although clearly decreased.
Specimen 1L: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: midbrain Strong 1L Expression particularly strong at E16-P0.
TS27: periaqueductal grey Strong 1L
TS27: pontine reticular formation Strong 1L
Specimen 1M: embryonic day 14.0 (more )
Structure Level Pattern Image Note
TS22: hindbrain Present Widespread 1M Widely expressed in most brain regions during development.
Specimen 1N: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: hypothalamus Strong 1N Expression remained high during postnatal development.
Specimen 1O: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: cerebellum Strong 1O Expression remained high during postnatal development.
Specimen 1P: postnatal adult (more )
Structure Level Pattern Image Note
TS28: brain Present 1P Expression still significant in adult, although clearly decreased.
TS28: cerebellum granule cell layer Strong 1P Strong expression in cerebellum, mostly in granular layer.
TS28: deep cerebellar nucleus Present 1P Signficant expression in deep nuclei.
Specimen S1A: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: hippocampus CA1 hippocampal pyramidal neuron Present Regionally restricted S1A Expression on apical dendrites of CA1 pyramidal neurons of stratum radiatum.
TS28: hippocampus stratum radiatum hippocampal pyramidal neuron Present Regionally restricted S1A Expression on apical dendrites of CA1 pyramidal neurons of stratum radiatum.
Specimen S1B: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: dentate gyrus granule cell layer dentate gyrus granule cell Present Regionally restricted S1B Expression around granular cells of dentate gyrus.
Specimen 5D,E: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: hypothalamus Present Regionally restricted 5E , 5D Expression in cells near arcuate nucleus.
Specimen 5H: postnatal day 15 (more )
Structure Level Pattern Image Note
TS28: corpus callosum Weak Regionally restricted 5H Expression in cells with typically elongated shape.
Specimen E14 not shown: embryonic day 14.0 (more )
Note: Data presented in Table 1.
Structure Level Pattern Image Note
TS22: hippocampus Very strong
TS22: rhombic lip Strong
TS22: thalamus Strong
TS22: brainstem nucleus Strong Regionally restricted Expression in olivary nuclei.
TS22: cortical subplate Present
TS22: epithalamus Present
TS22: globus pallidus Present
TS22: habenula Present
TS22: medullary raphe Present
TS22: preoptic area Present
TS22: red nucleus Present
TS22: subcommissural organ Present
TS22: midbrain Present Regionally restricted Expression in periaqueductal gray.
TS22: inferior colliculus Weak
TS22: superior colliculus Weak
TS22: brainstem Weak Regionally restricted Expression in reticular pontine nucleus
Specimen E16 not shown: embryonic day 16.0 (more )
Note: Data presented in Table 1.
Structure Level Pattern Image Note
TS24: hippocampus Very strong
TS24: main olfactory bulb mitral cell layer Very strong
TS24: cortical subplate Strong
TS24: epithalamus Strong
TS24: ganglionic eminence Strong
TS24: habenula Strong
TS24: subcommissural organ Strong
TS24: thalamus Strong
TS24: amygdala Strong Regionally restricted Expression in amygdaloid complex.
TS24: brainstem nucleus Strong Regionally restricted Expression in olivary nuclei.
TS24: globus pallidus Present
TS24: hypothalamus Present
TS24: main olfactory bulb prospective glomerular layer Present
TS24: main olfactory bulb prospective granule cell layer Present
TS24: red nucleus Present
TS24: midbrain Present Regionally restricted Expression in periaqueductal gray and raphe.
TS24: inferior colliculus Weak
TS24: superior colliculus Weak
TS24: cerebral cortex marginal layer Trace
Specimen E18 not shown: embryonic day 18.0 (more )
Note: Data presented in Table 1.
Structure Level Pattern Image Note
TS26: cortical plate Very strong
TS26: epithalamus Very strong
TS26: habenula Very strong
TS26: hippocampus CA1 Very strong
TS26: hippocampus CA2 Very strong
TS26: hippocampus CA3 Very strong
TS26: main olfactory bulb mitral cell layer Very strong
TS26: rhombic lip Very strong
TS26: subcommissural organ Very strong
TS26: cerebellum Very strong Regionally restricted Expression in cerebellum external germinal layer.
TS26: cerebellum Purkinje cell layer Strong
TS26: dentate gyrus Strong
TS26: main olfactory bulb prospective granule cell layer Strong
TS26: periaqueductal grey Strong
TS26: telencephalon septum Strong Regionally restricted Strong expression in dorso-lateral septum. Expression in medial septum/diagonal band.
TS26: hypothalamic nucleus Strong Regionally restricted Strong expression in ventral nuclei. Expression dorso-lateral nuclei and medial nuclei.
TS26: midbrain Strong Regionally restricted Expression in tegmental area and geniculate area. Low expression in raphe.
TS26: accumbens nucleus Present
TS26: caudate-putamen Present
TS26: cortical subplate Present
TS26: ganglionic eminence Present
TS26: globus pallidus Present
TS26: inferior colliculus Present
TS26: main olfactory bulb glomerular layer Present
TS26: piriform cortex Present
TS26: red nucleus Present
TS26: superior colliculus Present
TS26: telencephalon subventricular zone Present
TS26: amygdala Present Regionally restricted Expression in amygdaloid complex.
TS26: thalamic nucleus Present Regionally restricted Expression in dorso-lateral nuclei. Low expression in medial and ventral nuclei.
TS26: brainstem nucleus Present Regionally restricted Expression in olivary nuclei.
TS26: cerebral cortex marginal zone Trace
Specimen P0 not shown: postnatal day 0 (more )
Note: Data presented in Table 1.
Structure Level Pattern Image Note
TS27: cerebellum external granule cell layer Very strong
TS27: main olfactory bulb mitral cell layer Very strong
TS27: cerebellum Purkinje cell layer Strong
TS27: cortical plate Strong
TS27: epithalamus Strong
TS27: habenula Strong
TS27: hippocampus CA1 Strong
TS27: hippocampus CA2 Strong
TS27: hippocampus CA3 Strong
TS27: main olfactory bulb granule cell layer Strong
TS27: subcommissural organ Strong
TS27: telencephalon septum Strong Regionally restricted Strong expression in dorsolateral septum. Expression in medial septum/diagonal band.
TS27: thalamic nucleus Strong Regionally restricted Expression in dorso-lateral, medial and ventral thalamis nuclei.
TS27: midbrain Strong Regionally restricted Expression in tegmental and geniculate areas. Low expression in raphe.
TS27: corpus callosum Present
TS27: dentate gyrus Present
TS27: entorhinal cortex Present
TS27: inferior colliculus Present
TS27: main olfactory bulb glomerular layer Present
TS27: piriform cortex Present
TS27: superior colliculus Present
TS27: amygdala Present Regionally restricted Expression in amygdaloid complex.
TS27: hypothalamic nucleus Present Regionally restricted Expression in dorso-lateral, medial and ventral hypothalamic nuclei.
TS27: brainstem nucleus Present Regionally restricted Expression in olivary nuclei.
TS27: accumbens nucleus Weak
TS27: cortical subplate Weak
TS27: fimbria hippocampus Weak
TS27: globus pallidus Weak
TS27: red nucleus Weak
TS27: cerebral cortex marginal zone Trace
Specimen P5 not shown: postnatal day 5 (more )
Note: Data presented in Table 1.
Structure Level Pattern Image Note
TS28: main olfactory bulb mitral cell layer Very strong
TS28: cingulate cortex Strong
TS28: epithalamus Strong
TS28: habenula Strong
TS28: hippocampus CA1 Strong
TS28: hippocampus CA2 Strong
TS28: hippocampus CA3 Strong
TS28: main olfactory bulb granule cell layer Strong
TS28: retrosplenial cortex Strong
TS28: subcommissural organ Strong
TS28: cerebellum layer Strong Regionally restricted Authors report expression in external germinal layer.
TS28: cerebellum Purkinje cell layer Present
TS28: cortical layer II Present
TS28: cortical layer III Present
TS28: dentate gyrus Present
TS28: entorhinal cortex Present
TS28: main olfactory bulb glomerular layer Present
TS28: periaqueductal grey Present
TS28: piriform cortex Present
TS28: telencephalon subventricular zone Present
TS28: telencephalon septum Present Regionally restricted Expression in medial septum/diagonal band. Low expression in dorso-lateral septum.
TS28: amygdala Present Regionally restricted Expression in amygdaloid complex.
TS28: thalamic nucleus Present Regionally restricted Expression in dorso-lateral nuclei. Low expression i medial and ventral thalamic nuclei.
TS28: hypothalamic nucleus Present Regionally restricted Expression in ventral nuclei. Low expression in dorso-lateral and medial hypothalamic nuclei.
TS28: midbrain Present Regionally restricted Expression in tegmental and geniculate areas. Low expression in raphe.
TS28: accumbens nucleus Weak
TS28: caudate-putamen Weak
TS28: corpus callosum Weak
TS28: cortical layer V Weak
TS28: cortical layer VI Weak
TS28: globus pallidus Weak
TS28: inferior colliculus Weak
TS28: superior colliculus Weak
TS28: brainstem nucleus Weak Regionally restricted Low expression in olivary nuclei.
Specimen P10 not shown: postnatal day 10 (more )
Note: Data presented in Table 1.
Structure Cell Type Level Pattern Image Note
TS28: main olfactory bulb mitral cell layer Very strong
TS28: cingulate cortex Strong
TS28: dentate gyrus Strong
TS28: hippocampus CA1 Strong
TS28: hippocampus CA2 Strong
TS28: hippocampus CA3 Strong
TS28: main olfactory bulb granule cell layer Strong
TS28: retrosplenial cortex Strong
TS28: cerebellum layer Strong Regionally restricted Authors report expression in external germinal layer.
TS28: cerebellum Purkinje cell layer Present
TS28: cortical layer II Present
TS28: cortical layer III Present
TS28: cortical layer V Present
TS28: cortical layer VI Present
TS28: entorhinal cortex Present
TS28: epithalamus Present
TS28: habenula Present
TS28: main olfactory bulb glomerular layer Present
TS28: periaqueductal grey Present
TS28: piriform cortex Present
TS28: subcommissural organ Present
TS28: hippocampus hippocampal interneuron Present
TS28: telencephalon septum Present Regionally restricted Expression in medial septum/diagonal band. Low expression in dorsolateral septum.
TS28: thalamic nucleus Present Regionally restricted Expression in dorso-lateral, medial and ventral thalamic nuclei.
TS28: hypothalamic nucleus Present Regionally restricted Expression in ventral hypothalamic nuclei. Low expression in dorso-lateral and medial nuclei.
TS28: midbrain Present Regionally restricted Expression in tegmental and geniculate areas. Low expression in raphe.
TS28: accumbens nucleus Weak
TS28: caudate-putamen Weak
TS28: corpus callosum Weak
TS28: globus pallidus Weak
TS28: inferior colliculus Weak
TS28: superior colliculus Weak
TS28: telencephalon subventricular zone Weak
TS28: brainstem nucleus Weak Regionally restricted Low expression in olivary nuclei. Low expression in olivary nuclei.
TS28: amygdala Trace Regionally restricted Very low expression in amygdaloid comples.
TS28: fimbria hippocampus Absent
Specimen P15 not shown: postnatal day 15 (more )
Note: Data presented in Table 1.
Structure Cell Type Level Pattern Image Note
TS28: cerebellum granule cell layer Strong
TS28: cingulate cortex Strong
TS28: hippocampus CA1 Strong
TS28: hippocampus CA2 Strong
TS28: hippocampus CA3 Strong
TS28: main olfactory bulb granule cell layer Strong
TS28: main olfactory bulb mitral cell layer Strong
TS28: retrosplenial cortex Strong
TS28: cortical layer II Present
TS28: cortical layer III Present
TS28: dentate gyrus Present
TS28: entorhinal cortex Present
TS28: epithalamus Present
TS28: habenula Present
TS28: main olfactory bulb glomerular layer Present
TS28: periaqueductal grey Present
TS28: piriform cortex Present
TS28: subcommissural organ Present
TS28: telencephalon septum Present Regionally restricted Expression in medial septum/diagonal band. Low expression in dorso-lateral septum.
TS28: thalamic nucleus Present Regionally restricted Expression in dorso-lateral thalamic nuclei. Low expression in medial and ventral nuclei.
TS28: hypothalamic nucleus Present Regionally restricted Expression in ventral hypothalamic nuclei. Low expression in dorso-lateral and medial nuclei.
TS28: midbrain Present Regionally restricted Expression in tegmental and geniculate areas. Low expression in raphe.
TS28: accumbens nucleus Weak
TS28: caudate-putamen Weak
TS28: cerebellum Purkinje cell layer Weak
TS28: cortical layer V Weak
TS28: cortical layer VI Weak
TS28: globus pallidus Weak
TS28: inferior colliculus Weak
TS28: superior colliculus Weak
TS28: hippocampus hippocampal interneuron Weak
TS28: brainstem nucleus Weak Regionally restricted Low expression in olivary nuclei.
TS28: telencephalon subventricular zone Trace
TS28: cerebellum layer Trace Regionally restricted Authors report expression very low expression in external germinal layer
TS28: amygdala Trace Regionally restricted Very low expression in amygdaloid complex.
TS28: fimbria hippocampus Absent
Specimen P21 not shown: postnatal day 21 (more )
Note: Data presented in Table 1.
Structure Cell Type Level Pattern Image Note
TS28: main olfactory bulb mitral cell layer Very strong
TS28: cerebellum granule cell layer Present
TS28: cingulate cortex Present
TS28: dentate gyrus Present
TS28: entorhinal cortex Present
TS28: epithalamus Present
TS28: habenula Present
TS28: hippocampus CA1 Present
TS28: hippocampus CA2 Present
TS28: hippocampus CA3 Present
TS28: main olfactory bulb glomerular layer Present
TS28: main olfactory bulb granule cell layer Present
TS28: periaqueductal grey Present
TS28: piriform cortex Present
TS28: retrosplenial cortex Present
TS28: subcommissural organ Present
TS28: midbrain Present Regionally restricted Expression in tegmental and geniculate areas. Low expression in raphe.
TS28: accumbens nucleus Weak
TS28: caudate-putamen Weak
TS28: cerebellum Purkinje cell layer Weak
TS28: corpus callosum Weak
TS28: cortical layer II Weak
TS28: cortical layer III Weak
TS28: cortical layer V Weak
TS28: cortical layer VI Weak
TS28: globus pallidus Weak
TS28: inferior colliculus Weak
TS28: superior colliculus Weak
TS28: thalamic nucleus Weak Regionally restricted Low expression in dorso-lateral, medial and ventral thalamic nuclei.
TS28: hypothalamic nucleus Weak Regionally restricted Low expression in dorso-lateral, medial and ventral hypothalamic nuclei.
TS28: brainstem nucleus Weak Regionally restricted Low expression in olivary nuclei.
TS28: telencephalon subventricular zone Trace
TS28: hippocampus hippocampal interneuron Trace Very low expression in interneurons.
TS28: amygdala Trace Regionally restricted Very low expression in amygdaloid complex.
TS28: fimbria hippocampus Absent
Specimen Adult not shown: postnatal adult (more )
Note: Data presented in Table 1.
Structure Cell Type Level Pattern Image Note
TS28: main olfactory bulb mitral cell layer Strong
TS28: cingulate cortex Present
TS28: dentate gyrus Present
TS28: epithalamus Present
TS28: habenula Present
TS28: hippocampus CA1 Present
TS28: hippocampus CA2 Present
TS28: hippocampus CA3 Present
TS28: main olfactory bulb glomerular layer Present
TS28: main olfactory bulb granule cell layer Present
TS28: periaqueductal grey Present
TS28: retrosplenial cortex Present
TS28: subcommissural organ Present
TS28: thalamic nucleus Present Regionally restricted Expression in medial thalamic nuclei. Low expression in dorso-lateral and ventral nuclei.
TS28: midbrain Present Regionally restricted Expression in tegmental and geniculate areas. Low expression in rapher
TS28: accumbens nucleus Weak
TS28: caudate-putamen Weak
TS28: cerebellum Purkinje cell layer Weak
TS28: corpus callosum Weak
TS28: cortical layer II Weak
TS28: cortical layer III Weak
TS28: cortical layer V Weak
TS28: cortical layer VI Weak
TS28: globus pallidus Weak
TS28: inferior colliculus Weak
TS28: superior colliculus Weak
TS28: hypothalamic nucleus Weak Regionally restricted Low expression in dorso-lateral, medial and ventral hypothalamic nuclei.
TS28: brainstem nucleus Weak Regionally restricted Low expression in olivary nuclei.
TS28: telencephalon subventricular zone Trace
TS28: hippocampus hippocampal interneuron Trace
TS28: amygdala Trace Regionally restricted Very low expression in amygdaloid complex.
TS28: fimbria hippocampus Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory