Gene Expression Data
Assay Details
Assay
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Reference:
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J:111223
Kitano Y, et al., Gene expression of bone matrix proteins and endothelin receptors in endothelin-1-deficient mice revealed by in situ hybridization. J Bone Miner Res. 1998 Feb;13(2):237-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7859509
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Gene symbol:
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Spp1
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Gene name:
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secreted phosphoprotein 1
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Probe:
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OP cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 18.5; Edn1tm1Utj/Edn1 +
(more )
Specimen
5A:
(close )
Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: head mesenchyme |
Present |
Regionally restricted |
5A
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Expression is in mesenchymal cells close to craniofacial bones.
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TS26: mandible |
Present |
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5A
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Specimen
5B:
(close )
Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1tm1Utj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: head mesenchyme |
Present |
Regionally restricted |
5B
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Expression in this mutant is the same as in controls.
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TS26: mandible |
Present |
Regionally restricted |
5B
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Expression in this mutant is the same as in controls.
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