Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:111223
Kitano Y, et al., Gene expression of bone matrix proteins and endothelin receptors in endothelin-1-deficient mice revealed by in situ hybridization. J Bone Miner Res. 1998 Feb;13(2):237-44
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7859505
|
|
Gene symbol:
|
Sparc
|
|
Gene name:
|
secreted acidic cysteine rich glycoprotein
|
|
Probe:
|
ON cDNA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4A:
embryonic day 18.5; Edn1tm1Utj/Edn1 +
(more  )
Specimen
4A:
(close  )
|
Genetic Background:
|
involves: 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Edn1tm1Utj/Edn1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: head mesenchyme |
|
Strong |
Regionally restricted |
4A
|
Expression is in mesenchymal cells close to craniofacial bones.
|
| TS26: cranium |
odontoblast |
Strong |
|
4A
|
Expression is in odontoblasts.
|
Specimen
4B:
embryonic day 18.5; Edn1tm1Utj/Edn1 +
(more )
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Edn1tm1Utj/Edn1+
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: head mesenchyme |
|
Strong |
Regionally restricted |
4B
|
Expression is in mesenchymal cells close to craniofacial bones.
|
| TS26: cranium |
odontoblast |
Strong |
|
4B
|
Expression is in odontoblasts.
|
Specimen
4C:
embryonic day 16.5; Edn1tm1Utj/Edn1 +
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Edn1tm1Utj/Edn1+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4E:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Edn1tm1Utj/Edn1tm1Utj
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
4F:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Edn1tm1Utj/Edn1tm1Utj
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: head mesenchyme |
Present |
Regionally restricted |
4F
|
Expression in this mutant is the same as in controls.
|
| TS26: Meckel's cartilage |
Present |
|
4F
|
Expression in this mutant is the same as in controls.
|
Specimen
4G:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Edn1tm1Utj/Edn1tm1Utj
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools
