Gene Expression Data
Assay Details
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Assay
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Reference:
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J:111223
Kitano Y, et al., Gene expression of bone matrix proteins and endothelin receptors in endothelin-1-deficient mice revealed by in situ hybridization. J Bone Miner Res. 1998 Feb;13(2):237-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7859500
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Gene symbol:
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Ednra
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Gene name:
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endothelin receptor type A
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Probe:
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ETA cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 18.5; Edn1tm1Utj/Edn1 +
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: head mesenchyme |
Strong |
Regionally restricted |
2A
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Expression is in mesenchymal cells close to craniofacial bones.
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| TS26: mandible |
Present |
Scattered |
2A
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Expression is mainly positioning at the outer surface of bone matrix and at the inner region facing the teeth.
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Specimen
2B:
embryonic day 18.5; Edn1tm1Utj/Edn1 +
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: mandible |
Present |
Scattered |
2B
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Expression is mainly positioning at the outer surface of bone matrix and at the inner region facing the teeth.
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Specimen
2C:
embryonic day 18.5; Edn1tm1Utj/Edn1 +
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: cranium |
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Strong |
Non-Uniform |
2C
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Expression is stronger along the suture lines.
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| TS26: cranium |
odontoblast |
Strong |
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2C
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Expression is in odontoblasts.
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Specimen
2D:
embryonic day 16.5; Edn1tm1Utj/Edn1 +
(more )
Specimen
2D:
(close )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: head mesenchyme |
Weak |
Regionally restricted |
2D
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Expression is in mesenchymal cells near the bone.
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Specimen
2E:
(close )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1tm1Utj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cranium |
Present |
Regionally restricted |
2E
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In this mutant expression is in the cranio-facial bones but their arrangement varies from controls.
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1tm1Utj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cranium |
Present |
Regionally restricted |
2F
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In this mutant expression is in the cranio-facial bones but their arrangement varies from controls.
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1tm1Utj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cranium |
Present |
Regionally restricted |
2G
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In this mutant expression is in the cranio-facial bones but their arrangement varies from controls.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129X1/SvJ * ICR
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Mutant Allele(s):
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Edn1tm1Utj/Edn1tm1Utj
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: head mesenchyme |
Weak |
Regionally restricted |
2H
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Expression is in mesenchymal cells near the bone.
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