Antibody:	anti-ACF7
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Two antibodies were listed: MGI:4949158 and MGI:7857251, but the one used in this assay was not specified.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Widespread	1A E12.5 Macf1 , 1A E12.5 merge	MACF1 was broadly expressed in the developing cerebral cortex at E12.5, with higher levels in the ventricular zone and upper cortical areas near the marginal zone.
TS20: cerebral cortex ventricular layer	Present	Regionally restricted	1A E12.5 merged high mag , 1A E12.5 Macf1 , 1A E12.5 Macf1 high mag , 1A E12.5 merge	Higher expression levels were observed in the ventricular zone. Ventricular labeling was associated with nestin-positive radial glial neural progenitors.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Weak	Regionally restricted	1A E15.5 Macf1 high mag , 1A E15.5 merge , 1A E15.5 Macf1 , 1A E15.5 merged high mag	At E15.5, MACF1 expression at the ventricular zone was reduced compared to the expression pattern at E12.5.
TS23: cortical plate	Present	Regionally restricted	1A E15.5 merge , 1A E15.5 Macf1 , 1A E15.5 Macf1 high mag , 1A E15.5 merged high mag	Instead, MACF1 levels were higher in the cortical plate where postmitotic neurons were positioned.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cerebral cortex marginal layer	cerebral cortex neuron	Present		1B E12.5 merged high mag , 1B E12.5 Macf1 , 1B E12.5 Macf1 high mag , 1B E12.5 merge	The neuronal localization was shown by double immunostaining with a MAP2 antibody.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cortical plate	cerebral cortex neuron	Strong		1B E15.5 merged high mag , 1B E15.5 Macf1 high mag , 1B E15.5 merge , 1B E15.5 Macf1
TS23: cortical subplate	cerebral cortex neuron	Strong		1B E15.5 merge , 1B E15.5 Macf1