Gene Expression Data
Assay Details
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Assay
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Reference:
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J:245876
Rohacek AM, et al., ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development. Dev Cell. 2017 Nov 06;43(3):318-331.e5
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7857195
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Antibody:
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anti-Otx2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
5B:
embryonic day 16.5
(more  )
Specimen
5B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlea epithelium |
Present |
Regionally restricted |
5B
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Expression progressively restricted to Reissner's membrane.
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Specimen
5E:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Esrp1tm1.2Rpc/Esrp1tm1.2Rpc
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlea epithelium |
Present |
Regionally restricted |
5E
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Three-fold increase in number of expressing cells compared to control.
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Specimen
S6I:
embryonic day 13.5
(more )
Specimen
S6I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cochlea epithelium |
Present |
Regionally restricted |
S6I
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Remains broadly expressed throughout lateral cochlear epithelium.
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Specimen
S6J:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Esrp1tm1.2Rpc/Esrp1tm1.2Rpc
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cochlea epithelium |
Present |
Regionally restricted |
S6J
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Expression progressively restricted to Reissner's membrane.
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Specimen
S6K:
embryonic day 14.5
(more )
Specimen
S6K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea epithelium |
Present |
Regionally restricted |
S6K
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Remains broadly expressed throughout lateral cochlear epithelium.
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Specimen
S6L:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Esrp1tm1.2Rpc/Esrp1tm1.2Rpc
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea epithelium |
Present |
Regionally restricted |
S6L
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Remains broadly expressed throughout lateral cochlear epithelium.
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Specimen
S6M:
embryonic day 15.5
(more )
Specimen
S6M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea epithelium |
Present |
Regionally restricted |
S6M
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Expression progressively restricted to Reissner's membrane. Expression repressed from stria vascularis.
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Specimen
S6N:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Esrp1tm1.2Rpc/Esrp1tm1.2Rpc
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea epithelium |
Present |
Regionally restricted |
S6N
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Remains broadly expression throughout lateral cochlear epithelium.
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Specimen
7B:
embryonic day 18.5
(more )
Specimen
7B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7I:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Esrp1tm1.2Rpc/Esrp1tm1.2Rpc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlear duct |
Present |
Regionally restricted |
7I
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Expansion of Reissner's membrane.
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Specimen
7P:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Esrp1tm1.2Rpc/Esrp1tm1.2Rpc
Fgf9tm1b(KOMP)Wtsi/Fgf9+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlear duct |
Present |
Regionally restricted |
7P
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Signficant retraction of Reissner's membrane.
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