Antibody:	anti-alpha smooth muscle actin (ab7817)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: intestine smooth muscle longitudinal layer	Strong		1B Ednrb-/- ganglionic segment
TS28: intestine smooth muscle circular layer	Present		1B Ednrb-/- ganglionic segment

Structure	Level	Pattern	Image	Note
TS28: intestine smooth muscle circular layer	Strong		1B Ednrb-/- aganglionic segment
TS28: intestine smooth muscle longitudinal layer	Strong		1B Ednrb-/- aganglionic segment

Structure	Level	Pattern	Image	Note
TS28: intestine smooth muscle longitudinal layer	Strong		1B WT Proximal colon
TS28: intestine smooth muscle circular layer	Present		1B WT Proximal colon

Structure	Level	Pattern	Image	Note
TS28: intestine smooth muscle longitudinal layer	Strong		1B WT Distal colon
TS28: intestine smooth muscle circular layer	Present		1B WT Distal colon

Structure	Level	Pattern	Image	Note
TS20: gut muscularis	Present	Regionally restricted	3A E12.5	Expression is in the outer intestinal layers.

Structure	Level	Pattern	Image	Note
TS21: gut muscularis	Present		3A E13.5	Expression is in the outer intestinal layers.

Structure	Level	Pattern	Image	Note
TS23: gut muscularis	Present	Non-Uniform	3A E15.5	Expression is higher in the outer layer than the inner layer in the distal colon.

Structure	Level	Pattern	Image	Note
TS25: colon muscularis	Present	Non-Uniform	3A E17.5	Expression is higher in the outer layer than the inner layer in the distal colon.

Structure	Level	Pattern	Image	Note
TS26: colon muscularis	Present	Non-Uniform	3A E19.5	Expression is higher in the outer layer than the inner layer in the distal colon.
TS26: intestine smooth muscle longitudinal layer	Strong		3A E19.5
TS26: intestine smooth muscle circular layer	Present		3A E19.5

Structure	Level	Pattern	Image	Note
TS27: colon muscularis	Present	Non-Uniform	3A P1	Expression is higher in the outer layer than the inner layer in the distal colon.
TS27: intestine smooth muscle longitudinal layer	Strong		3A P1
TS27: intestine smooth muscle circular layer	Present		3A P1

Structure	Level	Pattern	Image	Note
TS28: colon muscularis	Present	Non-Uniform	3A P7	Expression is higher in the outer layer than the inner layer in the distal colon.
TS28: large intestine smooth muscle longitudinal layer	Strong		3A P7
TS28: large intestine smooth muscle circular layer	Present		3A P7

Structure	Level	Pattern	Image	Note
TS28: colon muscularis	Present	Non-Uniform	3A P21	Expression is higher in the outer layer than the inner layer in the distal colon.
TS28: large intestine smooth muscle longitudinal layer	Strong		3A P21
TS28: large intestine smooth muscle circular layer	Present		3A P21

Structure	Level	Pattern	Image	Note
TS20: gut muscularis	Present	Regionally restricted	3B E12.5	Expression is in the outer intestinal layers.

Structure	Level	Pattern	Image	Note
TS21: gut muscularis	Present	Regionally restricted	3B E13.5	Expression is in the outer intestinal layers.

Structure	Level	Pattern	Image	Note
TS23: gut muscularis	Present		3B E15.5	In this mutant there was was no statistically significant difference in expression between the inner and outer layers.

Structure	Level	Pattern	Image	Note
TS25: colon muscularis	Present		3b E17.5	In this mutant there was was no statistically significant difference in expression between the inner and outer layers and expression in the inner layer was higher than wild-type.

Structure	Level	Pattern	Image	Note
TS26: colon muscularis	Present		3B E19.5	In this mutant there was was no statistically significant difference in expression between the inner and outer layers and expression in the inner layer was higher than wild-type.

Structure	Level	Pattern	Image	Note
TS27: colon muscularis	Present		3B P1	In this mutant there was was no statistically significant difference in expression between the inner and outer layers and expression in the inner layer was higher than wild-type.

Structure	Level	Pattern	Image	Note
TS28: large intestine smooth muscle circular layer	Present		3B P7	In this mutant there was was no statistically significant difference in expression between the inner and outer layers and expression in the inner layer was higher than wild-type.
TS28: large intestine smooth muscle longitudinal layer	Present		3B P7	In this mutant there was was no statistically significant difference in expression between the inner and outer layers and expression in the inner layer was higher than wild-type.

Structure	Level	Pattern	Image	Note
TS28: large intestine smooth muscle circular layer	Present		3B P21	In this mutant there was was no statistically significant difference in expression between the inner and outer layers and expression in the inner layer was higher that seen in wild-type.
TS28: large intestine smooth muscle longitudinal layer	Present		3B P21	In this mutant there was was no statistically significant difference in expression between the inner and outer layers and expression in the inner layer was higher that seen in wild-type.