Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:149465
Holmes G, et al., Early onset of craniosynostosis in an Apert mouse model reveals critical features of this pathology. Dev Biol. 2009 Apr 15;328(2):273-84
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7856736
|
|
Gene symbol:
|
Spp1
|
|
Gene name:
|
secreted phosphoprotein 1
|
|
Probe:
|
Spp1 probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
2B WT:
embryonic day 15.5
(more  )
Specimen
2B WT:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: chondrocranium |
Present |
Regionally restricted |
2B WT Spp1
|
Expression in the coronal suture in the basal coronal suture very near or within regions of
fused osteoid.
|
Specimen
2B AP:
embryonic day 15.5; Fgfr2tm1Ewj/Fgfr2 +
(more )
Specimen
2B AP:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Fgfr2tm1Ewj/Fgfr2+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: chondrocranium |
Present |
Regionally restricted |
2B AP Spp1
|
Expression has increased in the coronal suture as the leading edges of expression extended
closer to each other.
|
Specimen
not shown WT E14.5:
embryonic day 14.5
(more )
Specimen
not shown WT E14.5:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium |
Present |
Regionally restricted |
|
Expression is deep within the osteogenic domains before progressing outwards towards the osteogenic fronts in the frontal and parietal bones.
|
Specimen
not shown AP E14.5:
embryonic day 14.5; Fgfr2tm1Ewj/Fgfr2 +
(more )
Specimen
not shown AP E14.5:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Fgfr2tm1Ewj/Fgfr2+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium |
Present |
Regionally restricted |
|
Expression in this mutant is the same as in wild-type.
|
Specimen
not shown AP E16.5:
embryonic day 16.5; Fgfr2tm1Ewj/Fgfr2 +
(more )
Specimen
not shown AP E16.5:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Fgfr2tm1Ewj/Fgfr2+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: chondrocranium |
Present |
Regionally restricted |
|
In this mutant there was continuous expression between frontal and parietal bones of the sutures.
|
|
Analysis Tools
