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Gene Expression Data
Assay Details
Assay
Reference: J:208419 Basta JM, et al., Sall1 balances self-renewal and differentiation of renal progenitor cells. Development. 2014 Mar;141(5):1047-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7855584
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Antibody: anti-Lef1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A E11.5 +/-: embryonic day 11.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Lef1; red - Ncam1 (assay MGI:7855585) and Ncam2 (assay MGI:7855586).
Structure Level Pattern Image Note
TS19: metanephros Present Regionally restricted 3A Lef1/NCAM - E11.5 +/- Lef1-positive;NCAM1/2-positive structures were observed with a morphologically distinct lumen.
Specimen 3A E12.5 -/-: embryonic day 12.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Lef1; red - Ncam1 (assay MGI:7855585) and Ncam2 (assay MGI:7855586).
Structure Level Pattern Image Note
TS20: metanephros Present Regionally restricted 3A Lef1/NCAM - E12.5 -/- There were significantly more Lef1-positive;NCAM1/2-positive structures with a morphologically distinct lumen in the mutant compared with control.
Specimen 3B +/+: embryonic day 13.5 (more )
Note: Double labeled: green - Lef1; red - Cytokeratin.
Structure Level Pattern Image Note
TS21: renal vesicle Present Regionally restricted 3B Lef1/Cyto +/+ Immunopositive renal vesicles formed on the ventral side of the ureter, towards the interior of the kidney.
Specimen 3B -/-: embryonic day 13.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Lef1; red - Cytokeratin.
Structure Level Pattern Image Note
TS21: renal vesicle Present Regionally restricted 3B Lef1/Cyto -/- In the mutant, some renal vesicles were located ectopically on the dorsal side of the ureter, towards the periphery of the forming kidney; these renal vesicle structures were immunopositive.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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