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Gene Expression Data
Assay Details
Assay
Reference: J:208419 Basta JM, et al., Sall1 balances self-renewal and differentiation of renal progenitor cells. Development. 2014 Mar;141(5):1047-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7855579
Gene symbol: Sall1
Gene name: spalt like transcription factor 1
Antibody: Anti-Sall1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The N-terminal antibody to Sall1 recognizes the 52 N-terminal amino acids fused to the GFP reporter.
Results
Specimen 2B E13.5 +/-: embryonic day 13.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Sall1; red - Cytokeratin.
Structure Level Pattern Image Note
TS21: cap mesenchyme Present 2B E13.5 +/-
TS21: renal vesicle Present 2B E13.5 +/-
TS21: renal interstitium Present Regionally restricted 2B E13.5 +/- Expression was detected in stroma.
TS21: renal tubule Present Regionally restricted 2B E13.5 +/- Expression was detected in differentiating tubule structures.
Specimen 2B E13.5 -/-: embryonic day 13.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Sall1; red - Cytokeratin.
Structure Level Pattern Image Note
TS21: renal tubule Present Regionally restricted 2B E13.5 -/- In the mutant expression was detected in ectopic renal vesicle/tubule-like structures, which were cytokeratin-negative.
Specimen 2B E14.5 +/-: embryonic day 14.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Sall1; red - Cytokeratin.
Structure Level Pattern Image Note
TS22: cap mesenchyme Present 2B E14.5 +/-
TS22: renal vesicle Present 2B E14.5 +/-
TS22: renal interstitium Present Regionally restricted 2B E14.5 +/- Expression was detected in stroma.
TS22: renal tubule Present Regionally restricted 2B E14.5 +/- Expression was detected in differentiating tubule structures.
Specimen 2B E14.5 -/-: embryonic day 14.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Sall1; red - Cytokeratin.
Structure Level Pattern Image Note
TS22: renal tubule Present Regionally restricted 2B E14.5 -/- In the mutant expression was detected in ectopic renal vesicle/tubule-like structures, which were cytokeratin-negative.
Specimen 2B E17.5 +/-: embryonic day 17.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Sall1; red - Cytokeratin.
Structure Level Pattern Image Note
TS25: cap mesenchyme Present 2B E17.5 +/- top
TS25: renal vesicle Present 2B E17.5 +/- top
TS25: renal interstitium Present Regionally restricted 2B E17.5 +/- top Expression was detected in stroma.
TS25: renal tubule Present Regionally restricted 2B E17.5 +/- top Expression was detected in differentiating tubule structures.
Specimen 2B E17.5 -/-: embryonic day 17.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Sall1; red - Cytokeratin.
Structure Level Pattern Image Note
TS25: renal tubule Present Regionally restricted 2B E17.5 -/- top In the mutant expression was detected in ectopic renal vesicle/tubule-like structures, which were cytokeratin-negative.
TS25: cap mesenchyme Absent 2B E17.5 -/- top
Specimen 3A E11.5 +/-: embryonic day 11.5; Sall1tm3Ryn/Sall1+ (more )
Structure Level Pattern Image Note
TS19: metanephros Present Regionally restricted 3A Sall1 - E11.5 +/- Control E11.5 heterozygous kidneys (matched to the mutant by the number of ureteric but tips - stage matched) had a small number of condensing cells forming pre-tubular aggregates.
Specimen 3A E12.5 -/-: embryonic day 12.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Structure Level Pattern Image Note
TS20: metanephros Present Regionally restricted 3A Sall1 - E12.5 -/- Expression was detected in numerous differentiating structures (pre-tubular aggregates or renal vesicles) that formed on the dorsal side of the ureter (ectopic location) in the E12.5 mutants.
Specimen 5E E12.5 +/-: embryonic day 12.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Sall1; red - pHH3.
Structure Level Pattern Image Note
TS20: metanephros Present 5E Sall1/pHH3 - E12.5 +/-
Specimen 5E E12.5 -/-: embryonic day 12.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Sall1; red - pHH3.
Structure Level Pattern Image Note
TS20: metanephros Present 5E Sall1/pHH3 - E12.5 -/-
Specimen 5E E13.5 +/-: embryonic day 13.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Sall1; red - pHH3.
Structure Level Pattern Image Note
TS21: metanephros Present 5E Sall1/pHH3 - E13.5 +/-
Specimen 5E E13.5 -/-: embryonic day 13.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Sall1; red - pHH3.
Structure Level Pattern Image Note
TS21: metanephros Present 5E Sall1/pHH3 - E13.5 -/- The mitotic index at E13.5 is significantly decreased in the mutant.
Specimen 5F E12.5 +/-: embryonic day 12.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Sall1; red - TUNEL.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 5F Sall1/TUNEL - E12.5 +/- Very few TUNEL+Sall1+ cells were observed.
Specimen 5F E12.5 -/-: embryonic day 12.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Sall1; red - TUNEL.
Structure Level Pattern Image Note
TS20: cap mesenchyme Present 5F Sall1/TUNEL - E12.5 -/- Very few TUNEL+Sall1+ cells were observed. No statistically significant difference in apoptotic index was detected at E12.5.
Specimen 5F E13.5 +/-: embryonic day 13.5; Sall1tm3Ryn/Sall1+ (more )
Note: Double labeled: green - Sall1; red - TUNEL.
Structure Level Pattern Image Note
TS21: cap mesenchyme Present 5F Sall1/TUNEL - E13.5 +/-
TS21: renal vesicle Present 5F Sall1/TUNEL - E13.5 +/-
Specimen 5F E13.5 -/-: embryonic day 13.5; Sall1tm3Ryn/Sall1tm3Ryn (more )
Note: Double labeled: green - Sall1; red - TUNEL.
Structure Level Pattern Image Note
TS21: cap mesenchyme Present 5F Sall1/TUNEL - E13.5 -/- Remaining cells in the cap mesenchyme of the mutant did not have significantly more TUNEL+ cells compared with control mice.
TS21: renal vesicle Present 5F Sall1/TUNEL - E13.5 -/- Significantly more cells were TUNEL+ at E13.5 in the renal vesicle formations of the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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