Antibody:	anti-Syntaxin 1
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The specificity of the antibody used was not detailed; both family members have been annotated.

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present	Regionally restricted	3B 1m WT Merge , 3B 1m WT Syntaxin1	Expression is in presynaptic nerves and nerve terminals.
TS28: hindlimb nerve	Present	Regionally restricted	3B 1m WT Merge , 3B 1m WT Syntaxin1	Expression is in presynaptic nerves and nerve terminals.

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present		3B 1m KO Merge , 3B 1m KO Syntaxin1	Staining indicates progressive denervation and altered morphology.
TS28: hindlimb nerve	Present		3B 1m KO Merge , 3B 1m KO Syntaxin1	Staining indicates progressive denervation and altered morphology.

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present		3B 4m WT Merge , 3B 4m WT Syntaxin1
TS28: hindlimb nerve	Present		3B 4m WT Merge , 3B 4m WT Syntaxin1

Structure	Level	Pattern	Image	Note
TS28: gastrocnemius muscle	Present		3B 4m KO Merge , 3B 4m KO Syntaxin1	Staining indicates progressive denervation and altered morphology.
TS28: hindlimb nerve	Present		3B 4m KO Merge , 3B 4m KO Syntaxin1	Staining indicates progressive denervation and altered morphology.

Structure	Level	Pattern	Image	Note
TS27: gastrocnemius muscle	Present	Regionally restricted	S3C WT Merge , S3C WT Syntaxin1	Expression is in motor axons and nerve terminals.
TS27: hindlimb nerve	Present	Regionally restricted	S3C WT Merge , S3C WT Syntaxin1	Expression is in motor axons and nerve terminals.

Structure	Level	Pattern	Image	Note
TS27: gastrocnemius muscle	Present		S3C KO Merge , S3C KO Syntaxin1	Expression in this mutant is the same as in wild-type.
TS27: hindlimb nerve	Present		S3C KO Merge , S3C KO Syntaxin1	Expression in this mutant is the same as in wild-type.