Gene Expression Data
Assay Details
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Assay
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Reference:
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J:123923
Zhou Q, et al., A multipotent progenitor domain guides pancreatic organogenesis. Dev Cell. 2007 Jul;13(1):103-14
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7852795
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Gene symbol:
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Cpa1
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Gene name:
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carboxypeptidase A1, pancreatic
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Probe:
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Cpa1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 14.5
(more  )
Note: Double labeled: reddish brown - Cpa1; purple - Myc (assay MGI:7852675).
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: reddish brown - Cpa1; purple - Myc (assay MGI:7852675).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: pancreas branching epithelium tip |
pancreas exocrine glandular cell |
Present |
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2A
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Exocrine cell marker. Expression overlaps completely with Myc staining.
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Specimen
2D:
embryonic day 14.5
(more )
Note: Double labeled: reddish brown - Cpa1; purple - Neurog3 (assay MGI:7852705).
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: reddish brown - Cpa1; purple - Neurog3 (assay MGI:7852705).
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Specimen
2G:
embryonic day 12.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas branching epithelium tip |
Strong |
Regionally restricted |
2G
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Expression was detected in the newly formed branches of the dorsal pancreas at E12.5. Expression was downregulated in the 'cleft' region between future branches.
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Specimen
2H:
embryonic day 12.5
(more )
Note: Double labeled: reddish brown - Cpa1; purple - Neurog3 (assay MGI:7852705).
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: reddish brown - Cpa1; purple - Neurog3 (assay MGI:7852705).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pancreas branching epithelium tip |
Present |
Regionally restricted |
2H
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Expression was detected in the newly formed branches of the dorsal pancreas at E12.5. Expression was downregulated in the 'cleft' region between future branches. Expression did not overlap with Neurog3 staining in the trunk.
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Specimen
S5A:
embryonic day 12.5
(more )
Note: Double labeled: reddish brown - Cpa1; purple - Myc (assay MGI:7852675).
Specimen
S5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: reddish brown - Cpa1; purple - Myc (assay MGI:7852675).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas branching epithelium tip |
Present |
Regionally restricted |
S5A
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Expression was detected in the newly formed branches of the dorsal pancreas at E12.5, where it co-expressed Myc. Expression was downregulated in the 'cleft' region between future branches.
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Specimen
S5B:
embryonic day 12.5
(more )
Note: Double labeled: reddish brown - Cpa1; purple - Ptf1a (assay MGI:7852679).
Specimen
S5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: reddish brown - Cpa1; purple - Ptf1a (assay MGI:7852679).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas branching epithelium tip |
Present |
Regionally restricted |
S5B
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Expression was detected in the newly formed branches of the dorsal pancreas at E12.5, where it co-expressed Ptf1a. Expression was downregulated in the 'cleft' region between future branches.
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Analysis Tools