Tnnt2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:350265 Feng H, et al., A new Prdm1-Cre line is suitable for studying the second heart field development. Dev Biol. 2024 Jun 14;514:78-86
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7852517
Gene symbol:	Tnnt2
Gene name:	troponin T2, cardiac

Antibody:	anti-cardiac Troponin T (26592-1-AP)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

2K ©

2K' ©

Specimen 2K,K': embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present		2K' , 2K

2L ©

2L' ©

Specimen 2L,L': embryonic day 9.5; Hand2^tm1.1Majh/Hand2^tm1.1Majh, Tg(Mef2c-cre)#Blk/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Absent		2L , 2L'	Expression lost in distal outflow tract.

2M ©

2M' ©

Specimen 2M,M': embryonic day 9.5; Hand2^tm1.1Majh/Hand2^tm1.1Majh, Prdm1^em1(cre)Zzy/Prdm1⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Absent		2M , 2M'	Expression lost in distal outflow tract.

3M ©

Specimen 3M: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Present		3M

3N ©

Specimen 3N: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present		3N
TS15: primitive ventricle	Present		3N

3O ©

Specimen 3O: embryonic day 9.5; Nkx2-5^em1Gpt/Nkx2-5^em1Gpt, Tg(Mef2c-cre)#Blk/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Weak		3O

3P ©

Specimen 3P: embryonic day 9.5; Nkx2-5^em1Gpt/Nkx2-5^em1Gpt, Tg(Mef2c-cre)#Blk/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Weak		3P
TS15: primitive ventricle	Weak		3P

3Q ©

Specimen 3Q: embryonic day 9.5; Nkx2-5^em1Gpt/Nkx2-5^em1Gpt, Prdm1^em1(cre)Zzy/Prdm1⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Present		3Q

3R ©

Specimen 3R: embryonic day 9.5; Nkx2-5^em1Gpt/Nkx2-5^em1Gpt, Prdm1^em1(cre)Zzy/Prdm1⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Weak		3R
TS15: primitive ventricle	Present		3R

5E ©

5E' ©

Specimen 5E,E': embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present		5E , 5E'

5F ©

5F' ©

Specimen 5F,F': embryonic day 9.5; Tbx20^em1Gpt/Tbx20^em1Gpt, Tg(Mef2c-cre)#Blk/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Weak		5F' , 5F

5G ©

5G' ©

Specimen 5G,G': embryonic day 9.5; Prdm1^em1(cre)Zzy/Prdm1⁺, Tbx20^em1Gpt/Tbx20^em1Gpt (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Weak		5G , 5G'	Outflow tract reduced in size compared to control.

5H ©

Specimen 5H: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present		5H

5I ©

Specimen 5I: embryonic day 9.5; Tbx20^em1Gpt/Tbx20^em1Gpt, Tg(Mef2c-cre)#Blk/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Weak		5I	Expression downregulated compared to control.

5J ©

Specimen 5J: embryonic day 9.5; Prdm1^em1(cre)Zzy/Prdm1⁺, Tbx20^em1Gpt/Tbx20^em1Gpt (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Weak		5J	Expression downregulated compared to control.

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