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Gene Expression Data
Assay Details
Assay
Reference: J:360528 Fritzsch B, et al., Irx3/5 Null Deletion in Mice Blocks Cochlea-Saccule Segregation and Disrupts the Auditory Tonotopic Map. J Comp Neurol. 2024 Dec;532(12):e70008
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7852162
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-beta-III Tubulin
Detection system: Secondary antibody
Results
Specimen 1A: embryonic day 16.5 (more )
Note: Double labeled: magenta - Tubb3; green - Myo7a (assay MGI:7852161).
Structure Level Pattern Image Note
TS24: inner ear Present 1A
Specimen 1B: embryonic day 16.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: Double labeled: magenta - Tubb3; green - Myo7a (assay MGI:7852161).
Structure Level Pattern Image Note
TS24: cochlea Weak 1B Expression in cochlea reduced compared to wild type.
TS24: cochlear ganglion Present Regionally restricted 1B Unusual shape of cochlear ganglion, forming two connectied entities: one with round shape near fused end-organ and second, more elongated ganglion in apex.
Specimen 1C,C': embryonic day 16.5 (more )
Note: Double labeled: magenta - Tubb3; green - Myo7a (assay MGI:7852161).
Structure Level Pattern Image Note
TS24: cochlea Present Regionally restricted 1C' , 1C Type I fibers reach inner hair cells, whereas type II fibers reach first row of outer hair cells.
TS24: saccule Present Regionally restricted 1C' , 1C Type I fibers reach inner hair cells, whereas type II fibers reach first row of outer hair cells.
Specimen 1C'': embryonic day 16.5 (more )
Note: Double labeled: magenta - Tubb3; green - Myo7a (assay MGI:7852161).
Structure Level Pattern Image Note
TS24: cochlea Present Regionally restricted 1C''
Specimen 1C''': embryonic day 16.5 (more )
Note: Double labeled: magenta - Tubb3; green - Myo7a (assay MGI:7852161).
Structure Level Pattern Image Note
TS24: cochlea Present Regionally restricted 1C''' Type I fibers reach inner hair cells, whereas type II fibers reach first row of outer hair cells.
Specimen 1D: embryonic day 16.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: Double labeled: magenta - Tubb3; green - Myo7a (assay MGI:7852161).
Structure Level Pattern Image Note
TS24: inner ear Weak 1D Expression in cochlea reduced compared to wild type.
Specimen 1D': embryonic day 16.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: Double labeled: magenta - Tubb3; green - Myo7a (assay MGI:7852161).
Structure Level Pattern Image Note
TS24: cochlea Weak Regionally restricted 1D' Fibers expand across cochlear basal turn.
TS24: saccule Weak Regionally restricted 1D' Fibers expand across cochlear basal turn.
Specimen 1D'': embryonic day 16.5; Irx3tm3.1Hui/Irx3tm3.1Hui, Irx5tm3Hui/Irx5tm3Hui (more )
Note: Double labeled: magenta - Tubb3.
Structure Level Pattern Image Note
TS24: cochlea Weak Regionally restricted 1D'' Apical hair cells have limited innervation.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory