Antibody:	anti-myosin VIIa (#25-6790)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Cell Type	Level	Pattern	Image	Note
TS24: inner ear	auditory hair cell	Present	Regionally restricted	1A	Expression in hair cells.
TS24: semicircular canal		Present	Regionally restricted	1A	Expression in cristae.
TS24: cochlea		Present		1A
TS24: saccule		Present		1A
TS24: utricle		Present		1A

Structure	Level	Pattern	Image	Note
TS24: semicircular canal	Present	Regionally restricted	1_Myo7A	Expression in cristae.
TS24: cochlea	Present		1_Myo7A
TS24: saccule	Present		1_Myo7A
TS24: utricle	Present		1_Myo7A

Structure	Level	Pattern	Image	Note
TS24: semicircular canal	Present		1B	Canal cristae and utricle comparable to wild-type.
TS24: utricle	Present		1B	Canal cristae and utricle comparable to wild-type.
TS24: saccule	Present		1B	Saccule continues across cochlea's basal turn.
TS24: organ of Corti	Present		1B	Organ of Corti length shortened compared to wild type.

Structure	Level	Pattern	Image	Note
TS24: cochlea	Present		1C' , 1C
TS24: saccule	Present		1C' , 1C

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cochlea	cochlear inner hair cell	Present		1C''	Single row of inner cells and 3 rows of outer hair cells.
TS24: cochlea	cochlear outer hair cell	Present		1C''	Single row of inner cells and 3 rows of outer hair cells.

Structure	Level	Pattern	Image	Note
TS24: cochlea	Present		1C'''	Single row of inner cells and 3 rows of outer hair cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: semicircular canal		Present		1D	Canal cristae and utricle comparable to wild-type.
TS24: utricle		Present		1D	Canal cristae and utricle comparable to wild-type.
TS24: saccule		Present		1D	Saccule continues across cochlea's basal turn.
TS24: organ of Corti		Present		1D	Organ of Corti length shortened compared to wild type.
TS24: cochlea	auditory hair cell	Present		1D	Ten or more rows of hair cells in fused cochlear base and saccule medial to organ of corti.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: saccule		Present		1D'
TS24: cochlea	auditory hair cell	Present		1D'	Ten or more rows of hair cells in fused cochlear base and saccule medial to organ of corti.

Structure	Level	Pattern	Image	Note
TS24: cochlea	Present		1D''