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Gene Expression Data
Assay Details
Assay
Reference: J:171050 Thiagarajan RD, et al., Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways. PLoS One. 2011;6(2):e17286
Assay type: RNA in situ
MGI Accession ID: MGI:7840423
Gene symbol: Spp2
Gene name: secreted phosphoprotein 2
Probe: RIKEN clone 0610038O04 subclone
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Authors described this gene as an 'anchor gene', which was defined as "genes whose expression is absolutely specific to a given anatomical compartment and/or developmental stage" in mouse kidney development.
Results
Specimen 2: embryonic day 15.5 (more )
Note: Includes data from Figure 5B.
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present 2_Spp2 Section in situ hybridization confirmed specific expression in early proximal tubule. Validated anchor gene. Expression was restricted to early proximal tubule of stage IV tubules.
Specimen S1Bc: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cortical early proximal tubule Present S1Bc , S1Bc' Expression was specific to the early proximal tubule (segment 1 & segment 2)
Specimen S2: postnatal adult (more )
Structure Level Pattern Image Note
TS28: proximal straight tubule Absent S2_Spp2 top , S2c
TS28: segment 2 of proximal tubule Present S2_Spp2 top , S2g
TS28: segment 1 of proximal tubule Present Regionally restricted S2g , S2_Spp2 top Expression was detected in a subset of segment 1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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