Smad4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316294 Hao XX, et al., Selective deletion of Smad4 in postnatal germ cells does not affect spermatogenesis or fertility in mice. Mol Reprod Dev. 2016 Jul;83(7):615-23
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7797284
Gene symbol:	Smad4
Gene name:	SMAD family member 4

Antibody:	anti-Smad4 (sc-7966)
Detection system:	Secondary antibody coupled to rhodamine

Results

1Aa ©

1Ab ©

Specimen 1Aa: postnatal adult (more

)

Structure	Cell Type	Level	Image
TS28: Leydig cell	Leydig cell	Present	1Ab , 1Aa
TS28: peritubular myoid cell	peritubular myoid cell	Present	1Ab , 1Aa
TS28: Sertoli cell	Sertoli cell	Present	1Aa , 1Ab
TS28: round spermatid		Present	1Ab , 1Aa
TS28: spermatocyte		Present	1Ab , 1Aa
TS28: elongated spermatid		Absent	1Aa , 1Ab

2G Smad4^fl/fl ©

Specimen 2G Smad4^fl/fl: postnatal adult; Smad4^tm2.1Cxd/Smad4^tm2.1Cxd (more

)

Structure	Level	Pattern	Image	Note
TS28: round spermatid	Present		2G Smad4^fl/fl
TS28: spermatocyte	Present		2G Smad4^fl/fl

Specimen 2G Smad4^fl/fl, Ngn3-Cre: postnatal adult; Smad4^tm2.1Cxd/Smad4^tm2.1Cxd, Tg(Neurog3-cre)C1Able/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: round spermatid	Absent		2G Smad4^fl/fl, Ngn3-Cre
TS28: spermatocyte	Absent		2G Smad4^fl/fl, Ngn3-Cre

Specimen 2H Smad4^fl/fl: postnatal adult; Smad4^tm2.1Cxd/Smad4^tm2.1Cxd (more

)

Structure	Level	Pattern	Image	Note
TS28: round spermatid	Present		2H Smad4^fl/fl
TS28: spermatocyte	Present		2H Smad4^fl/fl

Specimen 2G Smad4^fl/fl, Stra8-Cre: postnatal adult; Smad4^tm2.1Cxd/Smad4^tm2.1Cxd, Tg(Stra8-icre)1Reb/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: round spermatid	Absent		2H Smad4^fl/fl, Stra8-Cre
TS28: spermatocyte	Absent		2H Smad4^fl/fl, Stra8-Cre

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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