Probe:	Isl1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Cell Type	Level	Pattern	Image	Note
TS17: hindbrain	motor neuron	Present	Regionally restricted	1D	Expression is in motorneurons at the levels of rhombomeres 4-6.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: hindbrain	motor neuron	Present	Regionally restricted	1E	Expression is in motorneurons at the levels of rhombomeres 4-6.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain	motor neuron	Present	Regionally restricted	1F	Expression is in motorneurons at the levels of rhombomeres 4-6.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4A	Expression is in rhombomeres 4-6 and the facial branchiomotor neuron migration pathway.

Structure	Cell Type	Level	Pattern	Image	Note
TS21: hindbrain	motor neuron	Present	Regionally restricted	4B	Expression is in facial branchiomotor neurons in rhombomere 6.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4D	Expression is in rhombomere 4.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	4E	Expression indicates an abnormal distribution with an ectopic facial motor nucleus in dorsal rhombomere 4.
TS21: hindbrain floor plate	Present	Regionally restricted	4E	Expression is at the center.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4G	Expression is in rhombomere 4.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	4H	Expression indicates an abnormal distribution with an ectopic facial motor nucleus in dorsal rhombomere 4.
TS21: hindbrain floor plate	Present	Regionally restricted	4H	Expression is at the center.

Structure	Level	Pattern	Image	Note
TS21: facial nucleus	Present		4I
TS21: hindbrain	Present	Regionally restricted	4I	Expression is in rhombomere 6.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	4J	Expression is in rhombomere 6 and in a stream of cells in the dorsal region of rhombomere 5.
TS21: facial nucleus	Present		4J	The facial nucleus is less compact compared to wild-type.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	4K	Expression is in rhombomere 6 and in a stream of cells in the dorsal region of rhombomere 5.
TS21: facial nucleus	Present		4K	The facial nucleus is less compact compared to wild-type.

Structure	Level	Pattern	Image	Note
TS20: brain nucleus	Present	Regionally restricted	5D , 5E	Expression is in facial branchiomotor neurons.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	5H , 5G	Expression illustrates a partial defect in the migration, with divergent streams of migrating cells.
TS20: brain nucleus	Present	Regionally restricted	5G , 5H	The facial branchiomotor nucleus is less compact and extends into rhombomere 5 and 6.

Structure	Level	Pattern	Image	Note
TS20: brain nucleus	Present	Regionally restricted	5I	The facial branchiomotor nucleus is less compact and extends into rhombomere 5 and 6.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: hindbrain	motor neuron	Present	Regionally restricted	5J	Expression is in motorneurons at the levels of rhombomeres 4-6.

Structure	Level	Pattern	Image	Note
TS22: facial nucleus	Present		5K

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	5L	In this mutant the majority of facial branchiomotor neurons had failed to migrate caudally out of rhombomere 4 and remained close to the midline.

Structure	Level	Pattern	Image	Note
TS22: facial nucleus	Present		5M	The nucleus is reduced in size compared to wild-type.
TS22: hindbrain	Present	Regionally restricted	5M	There is expression in ectopic nuclei in dorsal rhombomeres 4 and 5.