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Gene Expression Data
Assay Details
Assay
Reference: J:305429 Kaiser M, et al., Regulation of otocyst patterning by Tbx2 and Tbx3 is required for inner ear morphogenesis in the mouse. Development. 2021 Apr 15;148(8):dev195651
Assay type: RNA in situ
MGI Accession ID: MGI:7788391
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Probe: Nefm probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 6A anterior control: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Present Regionally restricted 6A anterior control Nefm Expression was detected in the center of the statoacoustic ganglion.
Specimen 6A anterior Tbx2/3cDKO: embryonic day 10.5; Tbx2tm2.1Vmc/Tbx2tm2.1Vmc, Tbx3tm1Pa/Tbx3tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 6A anterior Tbx2/3cDKO Nefm Expression was unaltered in the Tbx2/3cDKO.
Specimen 6A mid-transverse control: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: otocyst Ambiguous 6A mid-transverse control Nefm
Specimen 6A mid-transverse Tbx2/3cDKO: embryonic day 10.5; Tbx2tm2.1Vmc/Tbx2tm2.1Vmc, Tbx3tm1Pa/Tbx3tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: otocyst Ambiguous 6A mid-transverse Tbx2/3cDKO Nefm
TS17: inner ear mesenchyme Present Single cells 6A mid-transverse Tbx2/3cDKO Nefm Some staining was seen in ectopic ganglion-like structures in the Tbx2/3cDKO periotic mesenchyme.
Specimen 6A posterior control: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: otocyst Ambiguous 6A posterior control Nefm
Specimen 6A posterior Tbx2/3cDKO: embryonic day 10.5; Tbx2tm2.1Vmc/Tbx2tm2.1Vmc, Tbx3tm1Pa/Tbx3tm1Pa, Tg(Pax2-cre)10Shwl/0, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: otocyst Ambiguous 6A posterior Tbx2/3cDKO Nefm
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory