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Gene Expression Data
Assay Details
Assay
Reference: J:184499 Lewis R, et al., Disruption of the developmentally-regulated Col2a1 pre-mRNA alternative splicing switch in a transgenic knock-in mouse model. Matrix Biol. 2012 Apr;31(3):214-26
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7787028
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Results
Specimen 5A: embryonic day 12.5 (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS20: hindlimb digit Present Regionally restricted 5A Expression is in the cartilaginous regions.
Specimen 5B: embryonic day 12.5; Col2a1tm1.1Mcali/Col2a1+ (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS20: hindlimb digit Present Regionally restricted 5B Expression is in the cartilaginous regions.
Specimen 5C: embryonic day 12.5; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS20: hindlimb digit Present Regionally restricted 5C Expression is in the cartilaginous regions.
Specimen 5D: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: tibia Present Regionally restricted 5D Expression is in the most proximal region in a "lacy" pattern in the proliferative and hypertrophic zones.
TS24: articular cartilage Present 5D
Specimen 5E: embryonic day 16.5; Col2a1tm1.1Mcali/Col2a1+ (more )
Structure Level Pattern Image Note
TS24: tibia Present Regionally restricted 5E Expression is in the most proximal region in a "lacy" pattern in the proliferative and hypertrophic zones.
TS24: articular cartilage Present 5E
Specimen 5F: embryonic day 16.5; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Structure Level Pattern Image Note
TS24: tibia Present Regionally restricted 5F In this mutant the "lacy" pattern was absent and there was continuing expression.
TS24: articular cartilage Present 5F
Specimen 5G: postnatal day 3 (more )
Structure Level Pattern Image Note
TS27: tibia Present Regionally restricted 5G Expression is in the most proximal region in a "lacy" pattern in the proliferative and hypertrophic zones.
TS27: articular cartilage Present 5G
Specimen 5H: postnatal day 3; Col2a1tm1.1Mcali/Col2a1+ (more )
Structure Level Pattern Image Note
TS27: tibia Present Regionally restricted 5H In this mutant the "lacy" pattern was absent and there was continuing expression.
TS27: articular cartilage Present 5H
Specimen 5I: postnatal day 3; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Structure Level Pattern Image Note
TS27: tibia Present Regionally restricted 5I In this mutant the "lacy" pattern was absent and there was continuing expression.
TS27: articular cartilage Present 5I
Specimen 5J: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 5J Expression is in the peripheral regions of developing articular cartilage.
TS28: articular cartilage Present 5J
Specimen 5K: postnatal day 7; Col2a1tm1.1Mcali/Col2a1+ (more )
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 5K In this mutant the "lacy" pattern was absent and there was continuing expression.
TS28: articular cartilage Present 5K
Specimen 5L: postnatal day 7; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 5L In this mutant the "lacy" pattern was absent and there was continuing expression.
TS28: tibia Present Regionally restricted 5L In this mutant the "lacy" pattern was absent and there was continuing expression.
Specimen 6A: postnatal day 14 (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 6A Expression is in the outermost region of the developing articular cartilage.
TS28: tibia Present Regionally restricted 6A Expression is in the outermost region of the developing articular cartilage.
TS28: trabecular bone Present Regionally restricted 6A Expression is in trabecular bone within the secondary center of ossification.
TS28: tibia metaphysis Present 6A
Specimen 6B: postnatal day 14; Col2a1tm1.1Mcali/Col2a1+ (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Strong 6B
TS28: tibia Strong 6B
TS28: trabecular bone Present Regionally restricted 6B Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral bone .
TS28: tibia metaphysis Present 6B
Specimen 6C: postnatal day 14; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Strong 6C
TS28: tibia Strong 6C
TS28: trabecular bone Present Regionally restricted 6C Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral bone .
TS28: tibia metaphysis Present 6C
Specimen 6D: postnatal day 28 (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 6G , 6D Expression is in the outermost region of the developing articular cartilage.
TS28: tibia Present Regionally restricted 6G , 6D Expression is in the outermost region of the developing articular cartilage.
TS28: trabecular bone Present Regionally restricted 6D , 6G Expression is in trabecular bone within the secondary center of ossification.
TS28: tibia metaphysis Present 6G , 6D
TS28: epiphyseal plate Absent 6G , 6D
Specimen 6E: postnatal day 28; Col2a1tm1.1Mcali/Col2a1+ (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 6E , 6H Expression is in and around articular chondrocytes.
TS28: tibia Present Regionally restricted 6E , 6H Expression is in and around articular chondrocytes.
TS28: epiphyseal plate Present 6H , 6E
TS28: tibia metaphysis Present 6H , 6E
TS28: trabecular bone Present Regionally restricted 6E , 6H Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral bone.
Specimen 6F: postnatal day 28; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 6I , 6F Expression is in and around articular chondrocytes.
TS28: tibia Present Regionally restricted 6I , 6F Expression is in and around articular chondrocytes.
TS28: epiphyseal plate Present 6I , 6F
TS28: tibia metaphysis Present 6I , 6F
TS28: trabecular bone Present Regionally restricted 6I , 6F Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral bone.
Specimen 6J: postnatal day 28 (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 6J Expression is in trabecular bone within the secondary center of ossification.
TS28: trabecular bone Present Regionally restricted 6J Expression is in trabecular bone within the secondary center of ossification.
TS28: tibia metaphysis Present 6J
Specimen 6K: postnatal day 28; Col2a1tm1.1Mcali/Col2a1+ (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 6K Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral and metaphyseal bone.
TS28: trabecular bone Present Regionally restricted 6K Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral and metaphyseal bone.
TS28: tibia metaphysis Present 6K
Specimen 6L: postnatal day 28; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: tibia Present Regionally restricted 6L Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral and metaphyseal bone.
TS28: trabecular bone Present Regionally restricted 6L Expression is in trabecular bone within the secondary center of ossification and in the trabeculae of the epiphyseal subchondral and metaphyseal bone.
TS28: tibia metaphysis Present 6L
Specimen 7A: postnatal day 70 (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Cell Type Level Pattern Image Note
TS28: articular cartilage articular chondrocyte Present Single cells 7A , 7D Expression is in the outermost region of articular cartilage and within chondrocytes of the deeper zones.
TS28: tibia articular chondrocyte Present Single cells 7A , 7D Expression is in the outermost region of articular cartilage and within chondrocytes of the deeper zones.
TS28: epiphyseal plate Absent 7A , 7D
TS28: trabecular bone Absent 7A , 7D
Specimen 7B: postnatal day 70; Col2a1tm1.1Mcali/Col2a1+ (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 7E , 7B Expression is along the periphery and in the deeper zones.
TS28: tibia Present Regionally restricted 7E , 7B Expression is along the periphery and in the deeper zones.
Specimen 7C: postnatal day 70; Col2a1tm1.1Mcali/Col2a1tm1.1Mcali (more )
Note: Double labeled: green - Col2a1 protein; red - Col2a1 protein (assay MGI:7787100).
Structure Level Pattern Image Note
TS28: articular cartilage Present Regionally restricted 7F , 7C Expression is along the periphery and in the deeper zones.
TS28: tibia Present Regionally restricted 7F , 7C Expression is along the periphery and in the deeper zones.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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