Col2a1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:184499 Lewis R, et al., Disruption of the developmentally-regulated Col2a1 pre-mRNA alternative splicing switch in a transgenic knock-in mouse model. Matrix Biol. 2012 Apr;31(3):214-26
Assay type:	RT-PCR
MGI Accession ID:	MGI:7787027
Gene symbol:	Col2a1
Gene name:	collagen, type II, alpha 1

Probe:	Col2a1-pF, Col2a1-pR
Probe preparation:	labelled with P32
Visualized with:	Autoradiography

Results

Image: 3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+/+	E12.5	TS20: hindlimb	Present (a)	Not Specified	Not Specified		Not Specified
ki/+	E12.5	TS20: hindlimb	Present (a)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified
ki/ki	E12.5	TS20: hindlimb	Present (a)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified
+/+	E16.5	TS24: hindlimb	Present (b)	Not Specified	Not Specified		Not Specified
ki/+	E16.5	TS24: hindlimb	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified
ki/ki	E16.5	TS24: hindlimb	Present (d)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified
+/+	P0	TS27: epiphyseal plate TS27: femur	Present (e)	Not Specified	Not Specified		Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/+	P0	TS27: epiphyseal plate TS27: femur	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/ki	P0	TS27: epiphyseal plate TS27: femur	Present (d)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified	Epiphyseal and femoral head cartilage tissue.
+/+	P3	TS27: epiphyseal plate TS27: femur	Present (e)	Not Specified	Not Specified		Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/+	P3	TS27: epiphyseal plate TS27: femur	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/ki	P3	TS27: epiphyseal plate TS27: femur	Present (d)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified	Epiphyseal and femoral head cartilage tissue.
+/+	P7	TS28: femur TS28: hindlimb skeleton	Present (e)	Not Specified	Not Specified		Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/+	P7	TS28: femur TS28: hindlimb skeleton	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/ki	P7	TS28: femur TS28: hindlimb skeleton	Present (d)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified	Epiphyseal and femoral head cartilage tissue.
+/+	P14	TS28: femur TS28: hindlimb skeleton	Present (e)	Not Specified	Not Specified		Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/+	P14	TS28: femur TS28: hindlimb skeleton	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/ki	P14	TS28: femur TS28: hindlimb skeleton	Present (d)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified	Epiphyseal and femoral head cartilage tissue.
+/+	P28	TS28: femur TS28: hindlimb skeleton	Present (e)	Not Specified	Not Specified		Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/+	P28	TS28: femur TS28: hindlimb skeleton	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/ki	P28	TS28: femur TS28: hindlimb skeleton	Present (d)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified	Epiphyseal and femoral head cartilage tissue.
+/+	P70	TS28: femur TS28: hindlimb skeleton	Present (e)	Not Specified	Not Specified		Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/+	P70	TS28: femur TS28: hindlimb skeleton	Present (c)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1⁺	Not Specified	Epiphyseal and femoral head cartilage tissue.
ki/ki	P70	TS28: femur TS28: hindlimb skeleton	Present (d)	Not Specified	involves: 129S6/SvEvTac * C57BL/6J * FVB/N	Col2a1^tm1.1Mcali/Col2a1^tm1.1Mcali	Not Specified	Epiphyseal and femoral head cartilage tissue.

Notes:
(a) This is the exon 2-containing isoform.
(b) There are two bands indicating the splice switch from IIA/IID to IIB mRNA.
(c) There are two bands corresponding to cDNA with or without exon 2.
(d) The IIA mRNA (exon 2-containing isoform) is the only transcript synthesized in this mutant.
(e) The IIB mRNA appears to be the major mRNA product produced at all post-natal time points.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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