Gene Expression Data
Assay Details
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Assay
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Reference:
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J:264500
Tsuchiya Y, et al., Ripply3 is required for the maintenance of epithelial sheets in the morphogenesis of pharyngeal pouches. Dev Growth Differ. 2018 Feb;60(2):87-96
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7786648
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Gene symbol:
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Itgb1
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Gene name:
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integrin beta 1 (fibronectin receptor beta)
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Antibody:
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9EG7
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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The antibody used recognizes the activated form of the protein.
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Results
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Specimen
4a:
embryonic day 9.0
(more  )
Note: 18-somite stage
Specimen
4a:
(close  )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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18-somite stage
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Specimen
4b/b':
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Ripply3tm1Sjt/Ripply3tm1Sjt
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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18-somite stage
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS14: 3rd branchial arch endoderm |
epithelial cell |
Present |
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4b
,
4b'
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Expression at the basal side of the endoderm cells was partly disturbed.
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| TS14: 3rd branchial arch mesenchyme |
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Present |
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4b
,
4b'
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Reduced mesenchymal staining due to a reduction in the number of mesenchymal cells in the presumptive 3rd pharyngeal arch.
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Specimen
4c:
embryonic day 9.5
(more )
Note: 24-somite stage
Specimen
4c:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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24-somite stage
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: 3rd branchial arch endoderm |
epithelial cell |
Present |
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4c
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Expression at the basal side of the endodermal epithelial cells became more evident.
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Specimen
4d:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Ripply3tm1Sjt/Ripply3tm1Sjt
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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24-somite stage
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Analysis Tools
