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Gene Expression Data
Assay Details
Assay
Reference: J:173838 Manuylov NL, et al., Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol. 2011 May 15;353(2):229-41
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7783713
Gene symbol: Dmrt1
Gene name: doublesex and mab-3 related transcription factor 1
Antibody: anti-DMRT1
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 5A control: embryonic day 13.5 (more )
Note: Double labeled: red - Dmrt1; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS21: germ cell of testis Present 5A right , 5A left
TS21: Sertoli cell Sertoli cell Present 5A left , 5A right
Specimen 5B GATA4cKO: embryonic day 13.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Dmrt1; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Trace 5B left , 5B right Virtually absent. Expression was dramatically downregulated.
TS21: germ cell of testis Present 5B left , 5B right Similar as in the control.
Specimen 5C control: embryonic day 17.5 (more )
Note: Double labeled: red - Dmrt1; green - Gata4 (assay MGI:7783737).
Structure Cell Type Level Pattern Image Note
TS25: Sertoli cell Sertoli cell Present 5C DMRT1 , 5C merge upper , 5C merge lower
TS25: germ cell of testis Present 5C merge lower , 5C merge upper , 5C DMRT1
Specimen 5D GATA4cKO: embryonic day 17.5; Gata4tm1.1Wtp/Gata4tm1.1Wtp, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Double labeled: red - Dmrt1; green - Gata4 (assay MGI:7783737).
Structure Cell Type Level Pattern Image Note
TS25: Sertoli cell Sertoli cell Trace 5D merge lower , 5D DMRT1 , 5D merge upper Virtually absent.
TS25: germ cell of testis Present 5D DMRT1 , 5D merge lower , 5D merge upper Similar as in the control.
Specimen 6A control: embryonic day 13.5 (more )
Note: Double labeled: red - Dmrt1; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 6A
TS21: germ cell of testis Present 6A
Specimen 6B FOG2cKO: embryonic day 13.5; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Double labeled: red - Dmrt1; green - Pecam1 (assay MGI:7782572).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 6B Similar as in the control.
TS21: germ cell of testis Present 6B Similar as in the control.
Specimen 6C FOG2null: embryonic day 13.5; Zfpm2tm1Sho/Zfpm2tm1Sho (more )
Note: Double labeled: red - Dmrt1; green - Pecam1 (assay MGI:7782572).
Structure Level Pattern Image Note
TS21: germ cell of testis Present 6C Similar as in the control.
TS21: testis Present Regionally restricted 6C Expression was lost in the somatic cells, due to a complete early block in Sertoli cell development.
Specimen 6D FOG2cKO: postnatal day 1; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Dmrt1; green - Gata4 (assay MGI:7782574).
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Present 6D
TS27: germ cell of gonad Present 6D
Specimen 6F/G FOG2cKO: postnatal day 1; Tg(Nr5a1-cre)2Klp/0, Zfpm2tm2Sho/Zfpm2tm2Sho (more )
Note: Conditional mutant. Hermaphrodite. Double labeled: red - Dmrt1; green - Gata4 (assay MGI:7782574).
Structure Cell Type Level Pattern Image Note
TS27: germ cell of gonad Present 6G , 6F
TS27: Sertoli cell Sertoli cell Present 6F , 6G
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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