Probe:	Zic1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: early migrating mesenchyme	Present	Regionally restricted	9C , 9B	Expression was detected in both layers of the early migrating mesenchyme (EMM). Table 1 ++ meningeal and ++ outer EMM.
TS20: supraorbital mesenchyme	Present	Regionally restricted	9D , 9B	Expression was detected in the meningeal layer of supraorbital mesenchyme (SOM). Table 1 ++ meningeal, (-) chodrogenic, (-) osteogenic, (-) dermal SOM.
TS20: diencephalon	Strong		9B
TS20: choroid plexus	Present		9B
TS20: cortical hem	Present		9B
TS20: telencephalon	Present	Regionally restricted	9B	Expression was detected in the hippocampus and the pallidum.

Structure	Level	Pattern	Image	Note
TS20: supraorbital mesenchyme	Present	Regionally restricted	9E	Expression was detected in the meningeal layer of supraorbital mesenchyme (SOM), and in the dermal layer at the parietal bone level. Zic1 expression was not found in the calvarial anlage within the SOM, which includes the osteogenic mesenchyme for the frontal bone and the parietal bone, and the prospective coronal suture in between.

Structure	Level	Pattern	Image	Note
TS20: early migrating mesenchyme	Present	Regionally restricted	S13B , S13A	Expression was detected in both layers of the early migrating mesenchyme (EMM). Table 1 ++ meningeal and ++ outer EMM.
TS20: supraorbital mesenchyme	Present	Regionally restricted	S13A , S13C	Expression was detected in the meningeal layer and in the dermal layer of supraorbital mesenchyme (SOM) at the parietal bone level. Table 1 ++ meningeal, (-) chodrogenic, (-) osteogenic, ++ dermal SOM.