Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:67359
Bruneau BG, et al., Cardiomyopathy in Irx4-deficient mice is preceded by abnormal ventricular gene expression. Mol Cell Biol. 2001 Mar;21(5):1730-6
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7781763
|
|
Gene symbol:
|
Irx5
|
|
Gene name:
|
Iroquois homeobox 5
|
|
Probe:
|
Irx5 probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4A:
embryonic day 9.5
(more  )
Specimen
4A:
(close  )
|
Genetic Background:
|
involves: 129X1/SvJ * Black Swiss
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart atrium |
Present |
|
4A
|
Robust expression in both atria and ventricles at E9.5 and E10.5.
|
| TS15: primitive ventricle |
Present |
|
4A
|
Robust expression in both atria and ventricles at E9.5 and E10.5.
|
| TS15: heart |
Present |
Regionally restricted |
4A
|
Expression excluded from atrioventricular junction.
|
Specimen
4B:
embryonic day 10.5
(more )
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * Black Swiss
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart atrium |
Present |
|
4B
|
Robust expression in both atria and ventricles at E9.5 and E10.5.
|
| TS17: primitive ventricle |
Present |
|
4B
|
Robust expression in both atria and ventricles at E9.5 and E10.5.
|
| TS17: heart |
Present |
Regionally restricted |
4B
|
Expression excluded from atrioventricular junction.
|
Specimen
4E:
embryonic day 10.5
(more )
Specimen
4E:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * Black Swiss
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart atrium |
Present |
|
4E
|
Robust expression in both atria and ventricles at E9.5 and E10.5.
|
| TS17: primitive ventricle |
Present |
|
4E
|
Robust expression in both atria and ventricles at E9.5 and E10.5.
|
| TS17: heart |
Present |
Regionally restricted |
4E
|
Expression excluded from atrioventricular junction.
|
Specimen
-/- not shown:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * Black Swiss
|
|
Mutant Allele(s):
|
Irx4tm1Jse/Irx4tm1Jse
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart |
Present |
Regionally restricted |
|
Expression not significanlty altered compared to wild type.
|
|
Analysis Tools