Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67359
Bruneau BG, et al., Cardiomyopathy in Irx4-deficient mice is preceded by abnormal ventricular gene expression. Mol Cell Biol. 2001 Mar;21(5):1730-6
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7781760
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Gene symbol:
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Hand1
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Gene name:
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heart and neural crest derivatives expressed 1
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Probe:
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eHAND probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2D +/+:
embryonic day 10.5
(more  )
Specimen
2D +/+:
(close  )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart |
Present |
Regionally restricted |
2D +/+
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Expression predominates in left ventricle and part of right ventricle.
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Specimen
2D -/-:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Irx4tm1Jse/Irx4tm1Jse
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: primitive ventricle |
Present |
Regionally restricted |
2D -/-
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Expression diminished in anterior and ventral regions of left ventricle.
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Specimen
2E +/+:
embryonic day 10.5
(more )
Specimen
2E +/+:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: primitive ventricle |
Present |
Regionally restricted |
2E +/+
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Expression predominates in left ventricle and part of right ventricle.
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Specimen
2E -/-:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Irx4tm1Jse/Irx4tm1Jse
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: primitive ventricle |
Present |
Regionally restricted |
2E -/-
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Expression diminished in anterior and ventral regions of left ventricle.
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Specimen
2F +/+:
embryonic day 10.5
(more )
Specimen
2F +/+:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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|
Age:
|
embryonic day 10.5
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|
Sex:
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Not Specified
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Type:
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whole mount
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|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: primitive ventricle |
Present |
Regionally restricted |
2F +/+
|
Expression predominates in left ventricle and part of right ventricle.
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Specimen
2F -/-:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Irx4tm1Jse/Irx4tm1Jse
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: primitive ventricle |
Present |
Regionally restricted |
2F -/-
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Expression diminished in anterior and ventral regions of left ventricle.
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Analysis Tools