Gene Expression Data
Assay Details
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Assay
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Reference:
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J:38996
Wroblewski J, et al., Distribution of nestin in the developing mouse limb bud in vivo and in micro-mass cultures of cells isolated from limb buds. Differentiation. 1997 Feb;61(3):151-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7780114
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Gene symbol:
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Des
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Gene name:
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desmin
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Antibody:
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anti-Desmin
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2D:
embryonic day 18.5
(more  )
Note: Double labeled: brown - Des protein; blue - Des mRNA (assay MGI:7780115).
Specimen
2D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Des protein; blue - Des mRNA (assay MGI:7780115).
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Specimen
not shown E13:
embryonic day 13.5
(more )
Specimen
not shown E13:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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3% Paraformaldehyde
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Analysis Tools
