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Gene Expression Data
Assay Details
Assay
Reference: J:38996 Wroblewski J, et al., Distribution of nestin in the developing mouse limb bud in vivo and in micro-mass cultures of cells isolated from limb buds. Differentiation. 1997 Feb;61(3):151-9
Assay type: RNA in situ
MGI Accession ID: MGI:7780112
Gene symbol: Nes
Gene name: nestin
Probe: Rat.401.gt10.16
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2A: embryonic day 12.5 (more )
Note: Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Regionally restricted 2A Expression is concentrated to the ends of the muscles, corresponding to the forming musculotendinous junctions.

Specimen 2B: embryonic day 12.5 (more )
Note: Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Regionally restricted 2B Expression is concentrated to the ends of the muscles, corresponding to the forming musculotendinous junctions.

Specimen 2C: embryonic day 12.5 (more )
Note: Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
Structure Level Pattern Image Note
TS20: forelimb mesenchyme Present Regionally restricted 2C Expression is concentrated to the ends of the muscles, corresponding to the forming musculotendinous junctions.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory