Gene Expression Data
Assay Details
Assay
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Reference:
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J:38996
Wroblewski J, et al., Distribution of nestin in the developing mouse limb bud in vivo and in micro-mass cultures of cells isolated from limb buds. Differentiation. 1997 Feb;61(3):151-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7780112
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Gene symbol:
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Nes
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Gene name:
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nestin
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Probe:
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Rat.401.gt10.16
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 12.5
(more )
Note: Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb mesenchyme |
Present |
Regionally restricted |
2A
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Expression is concentrated to the ends of the muscles, corresponding to the forming musculotendinous junctions.
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Specimen
2B:
embryonic day 12.5
(more )
Note: Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
Specimen
2B:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 12.5
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb mesenchyme |
Present |
Regionally restricted |
2B
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Expression is concentrated to the ends of the muscles, corresponding to the forming musculotendinous junctions.
|
Specimen
2C:
embryonic day 12.5
(more )
Note: Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Nes mRNA; brown - Nes protein (assay MGI:7780113).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb mesenchyme |
Present |
Regionally restricted |
2C
|
Expression is concentrated to the ends of the muscles, corresponding to the forming musculotendinous junctions.
|
|