Manf RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:142284 Lindholm P, et al., MANF is widely expressed in mammalian tissues and differently regulated after ischemic and epileptic insults in rodent brain. Mol Cell Neurosci. 2008 Nov;39(3):356-71
Assay type:	RNA in situ
MGI Accession ID:	MGI:7779865
Gene symbol:	Manf
Gene name:	mesencephalic astrocyte-derived neurotrophic factor

Probe:	Manf cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

Specimen 3A: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Strong		3A
TS28: main olfactory bulb mitral cell layer	Strong		3A

Specimen 3B: postnatal adult (more

)

Structure	Level	Image	Note
TS28: cortical layer II	Present	3B	relatively high
TS28: cortical layer III	Present	3B	relatively high
TS28: cortical layer IV	Present	3B	relatively high
TS28: cortical layer V	Present	3B	relatively high
TS28: cortical layer VI	Present	3B	relatively high
TS28: caudate-putamen	Moderate	3B

Specimen 3C: postnatal adult (more

)

Structure	Level	Image	Note
TS28: bed nucleus of stria terminalis	Strong	3C
TS28: paraventricular thalamic nucleus	Strong	3C
TS28: piriform cortex	Strong	3C
TS28: medial preoptic nucleus	Present	3C
TS28: choroid plexus	Strong	3C	Expressed in the non-neuronal cells.

Specimen 3D: postnatal adult (more

)

Structure	Level	Image
TS28: dentate gyrus granule cell layer	Strong	3D
TS28: dentate gyrus polymorphic layer	Strong	3D
TS28: hippocampus CA1	Strong	3D
TS28: hippocampus CA2	Strong	3D
TS28: hippocampus CA3	Strong	3D
TS28: dorsomedial hypothalamic nucleus	Strong	3D
TS28: ventromedial hypothalamic nucleus	Strong	3D

Specimen 3E: postnatal adult (more

)

Structure	Level	Image
TS28: dentate gyrus granule cell layer	Strong	3E
TS28: dentate gyrus polymorphic layer	Strong	3E
TS28: medial mammillary nucleus	Present	3E

Specimen 3F: postnatal adult (more

)

Structure	Level	Image
TS28: dentate gyrus granule cell layer	Strong	3F
TS28: dentate gyrus polymorphic layer	Strong	3F
TS28: substantia nigra	Moderate	3F

Specimen 3G: postnatal adult (more

)

Structure	Cell Type	Level	Image
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong	3G
TS28: facial nucleus		Present	3G
TS28: medial vestibular nucleus		Present	3G

Specimen 3H: postnatal adult (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Strong		3H

Specimen 3I: postnatal adult (more

)

Structure	Level	Image
TS28: dentate gyrus	Present	3I
TS28: hippocampus CA1	Present	3I
TS28: hippocampus CA2	Present	3I
TS28: hippocampus CA3	Present	3I

Specimen 3J: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3J	relatively high

Specimen 3K: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Strong		3K

Specimen 3L: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampal formation	Strong		3L

Specimen 3M: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: hippocampal formation	Strong		3M
TS27: cerebral cortex	Present		3M	relatively high

Specimen 3N: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		3N	relatively high

Specimen 4A: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Strong		4A

Specimen 4B: postnatal adult (more

)

Structure	Level	Image	Note
TS28: cortical layer II	Present	4B	relatively high
TS28: cortical layer III	Present	4B	relatively high
TS28: cortical layer IV	Present	4B	relatively high
TS28: cortical layer V	Present	4B	relatively high
TS28: cortical layer VI	Present	4B	relatively high

Specimen 4D: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Strong		4D

Specimen 4E: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Moderate		4E

Specimen 4F: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: bed nucleus of stria terminalis	Strong		4F
TS28: paraventricular thalamic nucleus	Strong		4F

Specimen 4G: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus polymorphic layer	Strong		4G

Specimen 4H: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Strong		4H

Specimen 4I: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA3	Strong		4I	especially intense

Specimen 4J: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Moderate		4J

Specimen 4K: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: locus coeruleus	Present		4K

Specimen 4L: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: pontine reticular nucleus	Present		4L

Specimen 5R: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		5R

Specimen 7A/B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	7A/B	Expressed in the roof.
TS20: corpus striatum	Present		7A/B	relatively low
TS20: dorsal root ganglion	Present		7A/B	relatively low
TS20: gut	Present		7A/B	relatively low
TS20: lung	Present		7A/B	relatively low
TS20: metanephros	Present		7A/B	relatively low
TS20: midbrain	Present		7A/B	relatively low
TS20: trigeminal ganglion	Present		7A/B	relatively low
TS20: head bone	Strong		7A/B	Expressed in the cartilage primordia.
TS20: liver	Strong		7A/B
TS20: umbilical blood vessel	Strong		7A/B
TS20: vertebral cartilage condensation	Strong		7A/B

Specimen 7C: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present		7C	Expressed in the roof.

Specimen 7D: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: lateral ventricle choroid plexus	Strong		7D	Expressed in non-neuronal cells.

Specimen 7E: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Strong		7E	relatively low

Specimen 7F: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: salivary gland	Strong		7F

Specimen not shown: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: embryo	Present			Widespread expression was detected.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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