Antibody:	anti-nestin antisera 130
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present	Patchy	1A Nes	There is widespread and patchy staining.
TS19: apical ectodermal ridge	Present		1A Nes

Structure	Cell Type	Level	Pattern	Image	Note
TS21: forelimb epithelium		Present		1B
TS21: forelimb nerve		Present		1B
TS21: forelimb mesenchyme		Present	Regionally restricted	1B	Expression is in islets of cells within the most proximal part.
TS21: blood vessel		Present	Regionally restricted	1B	Expression is in limb blood vessels.
TS21: forelimb digit mesenchyme		Weak	Regionally restricted	1B	Expression is in cells in the proximity of the forming cartilage phalanges and in subepithelial cells in the dermis.
TS21: forelimb cartilage condensation	chondroblast	Absent		1B	Expression is absent in differentiating chondroblasts and chondrocytes in the central parts of the limb bud.
TS21: forelimb cartilage condensation	chondrocyte	Absent		1B	Expression is absent in differentiating chondroblasts and chondrocytes in the central parts of the limb bud.

Structure	Level	Pattern	Image	Note
TS26: forelimb musculature	Weak		1C	Expression is in the Z bands.