Probe:	Rmst cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	1A	Expressed in the dorsal midline of the anterior neural axis. Expression in the dorsal midline extended to the anterior most tip of the telencephalon.
TS15: diencephalon	Present	Regionally restricted	1A	At the level of rostral diencephalon the expression domain expanded laterally into the alar plate and further to the basal plate at the caudal diencephalon. Expressed also in the caudal ventral region.
TS15: midbrain-hindbrain junction	Present		1A	A prominent band of expression was observed in the isthmus.
TS15: future midbrain floor plate	Present		1A
TS15: intermediate mesenchyme	Present		1A
TS15: telencephalon	Present	Regionally restricted	1A	Expressed dorsally.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	1B	Expressed in the dorsal midline of the anterior neural axis.
TS15: diencephalon	Present	Regionally restricted	1B	At the level of rostral diencephalon the expression domain expanded laterally into the alar plate and further to the basal plate at the caudal diencephalon.
TS15: midbrain-hindbrain junction	Present		1B	A prominent band of expression was observed in the isthmus.

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		1C	Similar as in the E9.5.
TS17: brain	Present	Regionally restricted	1C	Expressed in the dorsal midline, similar as in E9.5. Expression in the dorsal midline extended to the anterior most tip of the telencephalon.
TS17: telencephalon	Present	Regionally restricted	1C	Expressed dorsally.
TS17: diencephalon	Present	Regionally restricted	1C	Expressed in the rostral region. Expressed also in the caudal ventral region.
TS17: vascular plexus	Present	Regionally restricted	1C	Expressed in the ventral mesencephalic flexure where midbrain dopaminergic neurons are being generated.
TS17: midbrain floor plate	Present		1C

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	1D	Expressed in the rostral region. Expressed also in the caudal ventral region.
TS17: midbrain-hindbrain junction	Present		1D
TS17: telencephalon	Present	Regionally restricted	1D	Expression in the dorsal midline extended to the anterior most tip of the telencephalon.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2A	Expressed in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	2B	Expression extended to the alar plate. Expressed also in the caudal ventral region.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	2C	Expressed in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	2D	Expression was primarily restricted to the dorsal midline.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2E	Expressed in the dorsal midline.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	2F	Expressed dorsally. Expression extended to the alar plate.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	2G	Expression was primarily restricted to the dorsal midline. Expression in the ventral midbrain largely mirrors that of Lmx1a in both the progenitors in the ventricular and intermediate zone and in the mantle zone postmitotic cells.
TS17: midbrain floor plate	Present		2G
TS17: midbrain mantle layer	Present		2G
TS17: midbrain ventricular layer	Present		2G

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present	Regionally restricted	2H	Expression was primarily restricted to the dorsal midline.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	2I	Expression was primarily restricted to the dorsal midline. Expression was observed in the dorsal rhombic lip.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	2J	Expressed in the rostral spinal cord. Expression was primarily restricted to the dorsal midline.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Strong	Regionally restricted	3A	Expressed in both the roof plate and the floor plate, as well as in the most outer layer of cells in the alar plate and intermediate zone of the basal plate.
TS19: midbrain floor plate	Strong		3A	Expression level was high in the Lmx1a expressing region.
TS19: midbrain roof plate	Present		3A

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	3B	Expressed in both the roof plate and the floor plate, as well as in the most outer layer of cells in the alar plate and intermediate zone of the basal plate.
TS20: midbrain floor plate	Strong		3B	Expression level was high in the Lmx1a expressing region.
TS20: midbrain roof plate	Present		3B

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	3C	Expression in the ventral midbrain largely mirrors that of Lmx1a in both the progenitors in the ventricular and intermediate zone and in the mantle zone postmitotic cells.
TS19: midbrain mantle layer	Present		3C
TS19: midbrain ventricular layer	Present		3C
TS19: midbrain floor plate	Strong	Regionally restricted	3C	Expression level was high in the Lmx1a expressing region.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3D	Expression in the ventral midbrain largely mirrors that of Lmx1a in both the progenitors in the ventricular and intermediate zone and in the mantle zone postmitotic cells.
TS20: midbrain mantle layer	Present		3D
TS20: midbrain ventricular layer	Present		3D
TS20: midbrain floor plate	Strong		3D	Expression level was high in the Lmx1a expressing region.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	3E	Expression in the ventral midbrain largely mirrors that of Lmx1a in both the progenitors in the ventricular and intermediate zone and in the mantle zone postmitotic cells.
TS21: midbrain mantle layer	Present		3E
TS21: midbrain ventricular layer	Present		3E
TS21: midbrain floor plate	Strong		3E	Expression level was high in the Lmx1a expressing region.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	3F	Expression was primarily restricted to the forming ventral tegmental area that lies immediately adjacent to the ventral midline.
TS22: red nucleus	Present		3F