Gene Expression Data
Assay Details
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Assay
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Reference:
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J:175534
Sipe CW, et al., Kif3a regulates planar polarization of auditory hair cells through both ciliary and non-ciliary mechanisms. Development. 2011 Aug;138(16):3441-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7778317
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Gene symbol:
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Sptan1
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Gene name:
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spectrin alpha, non-erythrocytic 1
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Antibody:
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anti-alpha spectrin
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The specificity of the antibody used was not detailed; both family members have been annotated.
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Results
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Specimen
S1A:
embryonic day 18.5
(more  )
Specimen
S1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: utricle |
auditory hair cell |
Present |
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S1A
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Expression is in hair cells.
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Specimen
S1B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxg1tm1(cre)Skm/Foxg1+
Kif3atm2Gsn/Kif3atm2Gsn
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: utricle |
auditory hair cell |
Present |
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S1B
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In this mutant the polarity of hair cells and stereocilia organization is similar to controls.
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Analysis Tools
