Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: peripheral nervous system	Present		2A

Structure	Level	Pattern	Image	Note
TS15: peripheral nervous system	Present		2B	The positions and size of the cranial ganglia of the trigeminal, glossopharyngeal, and vagus nerves were almost normal. All the cranial nerves projected to their correct target regions. But the trajectory of each cranial nerve was severely disorganized in the mutant. No abnormal pathway or trajectory was observed in the oculomotor, trochlear, or vestibulocochlear nerves.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		2C

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		2D	The ophthalmic nerve was further defasciculated and widely spread in the frontal part. The distal parts of the maxillary andmandibular nerves were also defasciculated. The distal parts of the facial nerve was also expanded. No abnormal pathway or trajectory was observed in the oculomotor, trochlear, or vestibulocochlear nerves.

Structure	Level	Pattern	Image	Note
TS20: trigeminal nerve mandibular division	Present		2E
TS20: trigeminal nerve maxillary division	Present		2E
TS20: trigeminal nerve ophthalmic division	Present		2E

Structure	Level	Pattern	Image	Note
TS20: trigeminal nerve ophthalmic division	Present		2F	The ophthalmic nerve further expanded and overshot far beyond the growing front of the normal nerve within the front nasal part.
TS20: trigeminal nerve mandibular division	Present		2F	The nerve was defasciculated and spread into almost all parts.
TS20: trigeminal nerve maxillary division	Present		2F	The nerve was defasciculated and spread into almost all parts.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		3B	The patterned pathway and projection were severely disrupted in the mutant. Several positive fibers protruded directly from dorsal root ganglion. The spinal nerve fibers were globally separated into dorsal and ventral groups but never formed thick nerve fiber bundles.
TS17: dorsal root ganglion	Present		3B	Loose cell packaging was seen.

Structure	Level	Pattern	Image	Note
TS15: spinal ganglion or nerve	Present		3C	The pathway and projection of the spinal nerves were highly stereotyped and segmented.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		3D	Loose cell packaging was seen.
TS15: spinal ganglion or nerve	Present		3D	The main spinal nerve trunks were partially defasciculated. Several positive fibers left the main spinal nerve trunks at arbitrary points. The dorsal root afferent arrived at correct positions within the spinal cord and formed the dorsal funiculus.
TS15: spinal component of peripheral nervous system	Present		3D	The convergence of the spinal nerves into the branchial plexus was reduced.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		3E	The ramus dorsalis turned dorsolaterally and formed muscle branches to the dorsal muscle groups and skin sensory rami in a highly segmental pattern. The rami ventralis ran ventrally and sprouted the lateral cutaneous branches toward the lateral body wall.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		3F	Several positive fibers left the main spinal nerve trunks at arbitrary points. The dorsal root afferent arrived at correct positions within the spinal cord and formed the dorsal funiculus. Each spinal nerve fiber innervated the limbs almost independently.

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Present		3G	The ramus dorsalis turned dorsolaterally and formed muscle branches to the dorsal muscle groups and skin sensory rami in a highly segmental pattern. The rami ventralis ran ventrally and sprouted the lateral cutaneous branches toward the lateral body wall. No nerve fibers crossed the dorsal midline.

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Present		3H	Several positive fibers grew into the skin surface in an irregular manner. The segmental pattern of skin sensory innervation was partially but not completely disrupted. Several nerve fibers arrived at the opposite side of the embryos after crossing the dorsal midline. The dorsal root afferent arrived at correct positions within the spinal cord and formed the dorsal funiculus.

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Present		3I	Several nerve fibers arrived at the opposite side of the embryos after crossing the dorsal midline. The dorsal root afferent arrived at correct positions within the spinal cord and formed the dorsal funiculus.

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present		4A	The major dorsal and ventral nerve trunks grew further along particular routes within the limbs and sprouted skin sensory rami at discrete points.

Structure	Level	Pattern	Image	Note
TS20: forelimb nerve	Present		4B	Spinal nerves were distributedwidely within the forelimbs.

Structure	Level	Pattern	Image	Note
TS20: hindlimb nerve	Present		4C

Structure	Level	Pattern	Image	Note
TS20: hindlimb nerve	Present		4D	The trajectories of spinal nerve fibers toward the hind limbs were also disorganized and completely defaciculated within the limbs.
TS20: spinal nerve	Present		4D	The trajectories of spinal nerve fibers toward the hind limbs were also disorganized.

Structure	Level	Pattern	Image	Note
TS20: spinal nerve	Present			Spinal nerve fibers were poorly converged into either the crural or sciatic plexus.