Probe:	WISP-1 cDNA
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: cranium	Present	Regionally restricted	1A	Expressed in regions of mesenchymal condensations comprising flat bones of the skull destined to undergo intramembranous ossification.

Structure	Level	Pattern	Image	Note
TS23: vertebra	Strong	Regionally restricted	1B	Expression was high in osteoblasts and associated periosteal cells, and along the diaphysis forming the cortex of the long bone after ossification has begun. Expression was low or undetectable in chondrocytes and other cells surrounding osteogenic cells.

Structure	Level	Pattern	Image	Note
TS23: rib	Strong	Regionally restricted	1C	Expression was high in osteoblasts and associated periosteal cells, and along the diaphysis forming the cortex of the long bone after ossification has begun.

Structure	Level	Pattern	Image	Note
TS23: maxilla	Strong	Regionally restricted	1D	Expression was more prominent at sites of intramembranous ossification. The signal was predominantly in osteoblasts and periosteal cells. Expression was low or undetectable in chondrocytes and other cells surrounding osteogenic cells.

Structure	Level	Pattern	Image	Note
TS17: pre-cartilage condensation	Weak			Expressed in circumscribing precartilaginous condensations of developing endochondral bones.

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Present
TS20: skin	Present	Regionally restricted		Expressed in the subcutaneous mesenchyme.