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Gene Expression Data
Assay Details
Assay
Reference: J:171452 Holtz ML, et al., Serum response factor is required for cell contact maintenance but dispensable for proliferation in visceral yolk sac endothelium. BMC Dev Biol. 2011;11:18
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7750937
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Results
Specimen 2A: embryonic day 12.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Srf (assay MGI:7750935).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Strong 2A Used as a marker dependent on Srf for expression and was not used as a marker for the presence of smooth muscle cells.
Specimen 2B: embryonic day 12.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Srf (assay MGI:7750935).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Present 2B Expression remained but appeared decreased and disrupted in the mutant.
Specimen 3A: embryonic day 12.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Itga5 (assay MGI:7750938).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Strong 3A Acta2 was used to monitor Srf status in mesoderm due to antibody incompatibility between anti-Srf and anti-Itga5 antibodies.
Specimen 3B: embryonic day 12.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Itga5 (assay MGI:7750938).
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Present 3B Expression remained but appeared decreased and disrupted in the mutant.
Specimen S2A: embryonic day 10.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Srf (assay MGI:7750935).
Structure Level Pattern Image Note
TS17: yolk sac mesenchyme Present S2A
Specimen S2B: embryonic day 10.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Srf (assay MGI:7750935).
Structure Level Pattern Image Note
TS17: yolk sac mesenchyme Present S2B Expression remained but appeared decreased and disrupted in the mutant.
Specimen S2C: embryonic day 11.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Srf (assay MGI:7750935).
Structure Level Pattern Image Note
TS19: yolk sac mesenchyme Strong S2C
Specimen S2D: embryonic day 11.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - Srf (assay MGI:7750935).
Structure Level Pattern Image Note
TS19: yolk sac mesenchyme Present S2D Expression remained but appeared decreased and disrupted in the mutant.
Specimen S3A: embryonic day 12.5 (more )
Note: Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - pH3.
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Strong S3A
Specimen S3C: embryonic day 12.5; Srftm1Rmn/Srftm1Rmn, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Fixed in zinc fixative or zinc formalin. Double labeled: red - Acta2; green - pH3.
Structure Level Pattern Image Note
TS20: yolk sac mesenchyme Present S3C Expression remained but appeared decreased and disrupted in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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