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Gene Expression Data
Assay Details
Assay
Reference: J:298035 Kelly NH, et al., Single cell RNA-sequencing reveals cellular heterogeneity and trajectories of lineage specification during murine embryonic limb development. Matrix Biol. 2020 Jul;89:1-10
Assay type: RNA in situ
MGI Accession ID: MGI:7738315
Gene symbol: Col1a1
Gene name: collagen, type I, alpha 1
Probe: Col1a1 probe
Probe preparation: Antisense RNA
Assay notes: A grayscale version of the individual channels is provided in Supplemental Fig. 5.
Results
Specimen 5 E11.5: embryonic day 11.5 (more )
Note: Triple labeled: white - Col1a1; magenta - Runx2 (assay MGI:7738316); green - Scx (assay MGI:7738317).
Structure Level Pattern Image Note
TS19: footplate mesenchyme Absent 5E11.5 Co1a1 , 5E11.5 Merge No clear expression.
Specimen 5 E13.5: embryonic day 13.5 (more )
Note: Triple labeled: white - Col1a1; magenta - Runx2 (assay MGI:7738316); green - Scx (assay MGI:7738317).
Structure Level Pattern Image Note
TS21: hindlimb cartilage condensation Present 5E13.5 Col1a1 , 5E13.5 Merge Faint expression along the rays of the cartilaginous condensations.
Specimen 5 E15.5: embryonic day 15.5 (more )
Note: Triple labeled: white - Col1a1; magenta - Runx2 (assay MGI:7738316); green - Scx (assay MGI:7738317).
Structure Level Pattern Image Note
TS23: hindlimb cartilage condensation Present 5E15.5 Col1a1 , 5E15.5 Merge
Specimen 5 E18.5: embryonic day 18.5 (more )
Note: Triple labeled: white - Col1a1; magenta - Runx2 (assay MGI:7738316); green - Scx (assay MGI:7738317).
Structure Level Pattern Image Note
TS26: hindlimb cartilage condensation Present 5E18.5 Col1a1 , 5E18.5 Merge Expressed on bone that overlapped with Runx2, and along the sides of the bone, possibly marking tendon.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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