Antibody:	anti-Desmoglein 1/2 (clone DG 3.10, 61002)
Detection system:	Secondary antibody coupled to Cy3
Assay notes:	The specificity of the antibody used was not detailed; both family members have been annotated.

Structure	Level	Pattern	Image	Note
TS21: gubernaculum dentis	Present	Regionally restricted	4A	Expressed in the lateral part.
TS21: oral epithelium	Present	Regionally restricted	4A	Expression was much weaker in the medial part.
TS21: lower jaw molar epithelium	Present		4A	Expressed in the whole bud.

Structure	Level	Pattern	Image	Note
TS22: gubernaculum dentis	Present	Regionally restricted	4B	Expressed in the lateral part.
TS22: oral epithelium	Present	Regionally restricted	4B	Expression was much weaker in the medial part.
TS22: inner enamel epithelium	Absent		4B
TS22: outer enamel epithelium	Absent		4B

Structure	Level	Pattern	Image	Note
TS23: gubernaculum dentis	Present	Regionally restricted	4C	Expressed in the lateral part.
TS23: oral epithelium	Present	Regionally restricted	4C	Expression was much weaker in the medial part.
TS23: stellate reticulum	Weak	Regionally restricted	4C	Some cells showed a faint staining.
TS23: stratum intermedium	Weak	Regionally restricted	4C	Some cells showed a faint staining.
TS23: inner enamel epithelium	Absent		4C
TS23: outer enamel epithelium	Absent		4C

Structure	Level	Pattern	Image	Note
TS24: stellate reticulum	Present		4D
TS24: stratum intermedium	Present		4D	Stronger expression.
TS24: inner enamel epithelium	Absent		4D
TS24: outer enamel epithelium	Absent		4D

Structure	Level	Pattern	Image	Note
TS26: stellate reticulum	Present		4E
TS26: stratum intermedium	Present		4E	Stronger expression.
TS26: inner enamel epithelium	Absent		4E
TS26: outer enamel epithelium	Absent		4E

Structure	Cell Type	Level	Pattern	Image	Note
TS26: stellate reticulum		Ambiguous		4F	Decreased expression. Trace or absent.
TS26: oral epithelium		Present		4F
TS26: stratum intermedium		Strong		4F
TS26: gubernaculum dentis		Present		4F
TS26: inner enamel epithelium	ameloblast	Present	Regionally restricted	4F	Expressed at the basal poles of ameloblasts. Faint staining could also be detected in some areas of the inner dental epithelium.