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Gene Expression Data
Assay Details
Assay
Reference: J:170671 Shibata M, et al., MicroRNA-9 regulates neurogenesis in mouse telencephalon by targeting multiple transcription factors. J Neurosci. 2011 Mar 2;31(9):3407-22
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7736602
Gene symbol: Mki67
Gene name: antigen identified by monoclonal antibody Ki 67
Antibody: anti-Ki-67
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 5A miR-9-2 +/+; miR9-3 +/+: embryonic day 15.5 (more )
Note: Double labeled: green - Mki67; red - BrdU.
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present Regionally restricted 5A E15.5 BrdU/Ki67 top Mki67 expression was detected in cycling cells in the pallium.
TS23: cerebral cortex ventricular layer Present Regionally restricted 5A E15.5 BrdU/Ki67 top Mki67 expression was detected in cycling cells in the pallium.
Specimen 5A miR-9-2 -/-; miR9-3 -/-: embryonic day 15.5; Mir9-2tm1Sia/Mir9-2tm1Sia, Mir9-3tm1Sia/Mir9-3tm1Sia (more )
Note: Double labeled: green - Mki67; red - BrdU.
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present 5A E15.5 BrdU/Ki67 bottom The number of cycling Mki67-positive cells was significantly reduced in the double-mutant subventricular zone.
TS23: cerebral cortex ventricular layer Present 5A E15.5 BrdU/Ki67 bottom
Specimen 5E miR-9-2 +/+; miR9-3 +/+: embryonic day 16.5 (more )
Note: Double labeled: green - Mki67; red - BrdU.
Structure Level Pattern Image Note
TS24: cerebral cortex ventricular layer Present 5E top Mki67 expression was detected in cycling cells in the pallium.
Specimen 5E miR-9-2 -/-; miR9-3 -/-: embryonic day 16.5; Mir9-2tm1Sia/Mir9-2tm1Sia, Mir9-3tm1Sia/Mir9-3tm1Sia (more )
Note: Double labeled: green - Mki67; red - BrdU.
Structure Level Pattern Image Note
TS24: cerebral cortex ventricular layer Present 5E bottom The frequency of the cells reentering the cell cycle is given as the ratio of BrdU and Ki67 double-positive cells over the total number of BrdU-positive cells. This ratio was significantly reduced in the miR-9-2/3 double-mutant ventricular zone.
Specimen 5G miR-9-2 +/+; miR9-3 +/+ E17.5: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: cerebral cortex ventricular layer Present 5G E17.5 top Mki67 expression was detected in cycling cells in the pallium.
Specimen 5G miR-9-2 -/-; miR9-3 -/- E17.5: embryonic day 17.5; Mir9-2tm1Sia/Mir9-2tm1Sia, Mir9-3tm1Sia/Mir9-3tm1Sia (more )
Structure Level Pattern Image Note
TS25: cerebral cortex ventricular layer Present 5G E17.5 bottom The number of Mki67-positive proliferating cells in the ventricular zone significantly decreased at E17.5 and E18.5.
Specimen 5G miR-9-2 +/+; miR9-3 +/+ E18.5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebral cortex ventricular layer Present 5G E18.5 top Mki67 expression was detected in cycling cells in the pallium.
Specimen 5G miR-9-2 -/-; miR9-3 -/- E18.5: embryonic day 18.5; Mir9-2tm1Sia/Mir9-2tm1Sia, Mir9-3tm1Sia/Mir9-3tm1Sia (more )
Structure Level Pattern Image Note
TS26: cerebral cortex ventricular layer Present 5G E18.5 bottom The number of Mki67-positive proliferating cells in the ventricular zone significantly decreased at E17.5 and E18.5.
Specimen 9M': embryonic day 15.5 (more )
Note: Double labeled: green - Mki67; red - BrdU.
Structure Level Pattern Image Note
TS23: lateral ganglionic eminence Present Regionally restricted 9M' Mki67 expression was detected in cycling cells.
Specimen 9N': embryonic day 15.5; Mir9-2tm1Sia/Mir9-2tm1Sia, Mir9-3tm1Sia/Mir9-3tm1Sia (more )
Note: Double labeled: green - Mki67; red - BrdU.
Structure Level Pattern Image Note
TS23: lateral ganglionic eminence Present Regionally restricted 9N' The number of Mki67-positive cells was increased in the double mutant, particularly in the deeper area of the lateral ganglionic eminence.
Specimen S7C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: lateral ganglionic eminence Present Regionally restricted S7C Mki67 expression was detected in cycling cells.
Specimen S7D: embryonic day 12.5; Mir9-2tm1Sia/Mir9-2tm1Sia, Mir9-3tm1Sia/Mir9-3tm1Sia (more )
Structure Level Pattern Image Note
TS20: lateral ganglionic eminence Present Regionally restricted S7D There was no increase in cell proliferation in E12.5 miR-9-2/3 double mutant lateral ganglionic eminence.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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