Antibody:	RMO-270
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: vagal nerve trunk	Present		2d	To illustrate the exact location of the vagal nerve beside the fourth arch artery.

Structure	Level	Pattern	Image	Note
TS20: vagus nerve	Present		3f	The ring of non-actin expressing cells between the vagal and recurrent nerves was seen to be clearly interspersed with a maze of thin nerve fibers.

Structure	Level	Pattern	Image	Note
TS23: vagus nerve	Present		4b , 4c	The ring of non-actin expressing cells between the vagal and recurrent nerves was seen to be clearly interspersed with a maze of thin nerve fibers.

Structure	Level	Pattern	Image	Note
TS23: vagus nerve	Present		4d	The upper edge of the ring-formed nerve fibre network within the aortic wall was shown.

Structure	Level	Pattern	Image	Note
TS28: arch of aorta	Present	Regionally restricted	7b	Some of those small number of Acta2-negative cells in the B-segment, particularly those in the outer media, were positive for Nefm.

Structure	Level	Pattern	Image	Note
TS23: vagus nerve	Present	Regionally restricted		The presence of nerve fibres was clearly in the proximal right subclavian artery. Staining was never observed elsewhere in the media of the embryonic thoracic arteries.