Gene Expression Data
Assay Details
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Assay
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Reference:
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J:69569
DiPersio CM, et al., alpha3beta1 Integrin is required for normal development of the epidermal basement membrane. J Cell Biol. 1997 May 5;137(3):729-42
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7736211
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Gene symbol:
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Lama5
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Gene name:
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laminin, alpha 5
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Antibody:
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anti-laminin 5
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein
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Results
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Specimen
2D:
(close  )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: limb skin |
Present |
Regionally restricted |
2D
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Expression is at the dermal and epidermal sides of the blister and in what appeared to be remnants of matrix extending through the blisters.
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Specimen
3C:
postnatal newborn
(more  )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: basement membrane |
Present |
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3C
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Expression is in the basement membrane directly below the basal keratinocytes of the epidermis.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: basement membrane |
Present |
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3D
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In this mutant staining is disorganized compared to wild-type.
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Specimen
4B:
postnatal newborn
(more )
Note: Double labeled with Itga6 (assay MGI:7736215).
Specimen
4B:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with Itga6 (assay MGI:7736215).
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with Itga6 (assay MGI:7736215).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: basement membrane |
Present |
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4F
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In this mutant staining is disorganized compared to wild-type.
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Specimen
4J:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled with Itga6 (assay MGI:7736215).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: basement membrane |
Present |
Regionally restricted |
4J
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Expression is at the dermal and epidermal sides of the blister.
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| TS27: limb skin |
Present |
Regionally restricted |
4J
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Expression is at the dermal and epidermal sides of the blister.
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Specimen
7B:
embryonic day 15.5
(more )
Note: Less stratified region.
Specimen
7B:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Note:
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Less stratified region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: basement membrane |
Present |
Regionally restricted |
7B
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Expression is in the basement membrane zone in more stratified regions and is absent in less stratified regions (shown).
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| TS23: skin |
Present |
Regionally restricted |
7B
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Expression is in the basement membrane zone in more stratified regions and is absent in less stratified regions (shown).
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Specimen
7D:
embryonic day 15.5
(more )
Note: More stratified region.
Specimen
7D:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Note:
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More stratified region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: basement membrane |
Present |
Regionally restricted |
7D
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Expression is in the basement membrane zone in more stratified regions (shown) and is absent in less stratified regions.
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| TS23: skin |
Present |
Regionally restricted |
7D
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Expression is in the basement membrane zone in more stratified regions (shown) and is absent in less stratified regions.
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Specimen
7H:
embryonic day 17.5; Itga3tm1Jak/Itga3 +
(more )
Specimen
7H:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Specimen
7F:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Specimen
7J:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: basement membrane |
Present |
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7J
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In this mutant staining is disorganized compared to wild-type.
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| TS25: skin |
Present |
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7J
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In this mutant staining is disorganized compared to wild-type.
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Specimen
7L:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3tm1Jak
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: basement membrane |
Present |
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7L
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In this mutant staining is disorganized compared to wild-type.
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| TS25: skin |
Present |
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7L
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In this mutant staining is disorganized compared to wild-type.
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Specimen
not shown newborn:
postnatal newborn; Itga3tm1Jak/Itga3 +
(more )
Specimen
not shown newborn:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Itga3tm1Jak/Itga3+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: basement membrane |
Present |
Regionally restricted |
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In heterozygous mice levels were reduced but still easily detectable.
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Specimen
not shown E11.5:
embryonic day 11.5
(more )
Specimen
not shown E11.5:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Analysis Tools