Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49434
Farinas I, et al., Characterization of neurotrophin and Trk receptor functions in developing sensory ganglia: direct NT-3 activation of TrkB neurons in vivo. Neuron. 1998 Aug;21(2):325-34
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7735227
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Gene symbol:
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Ntrk2
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Gene name:
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neurotrophic tyrosine kinase, receptor, type 2
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Antibody:
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anti-TrkB
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1C:
embryonic day 11.0
(more  )
Note: Summarized in Table 1.
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Summarized in Table 1.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: dorsal root ganglion |
Present |
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1C
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Expressed in both intracellular and surface membrane compartments in axons as well as cell bodies of neurons. Expressed in a spatially skewed population of neurons.
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Specimen
1D:
embryonic day 13.0
(more )
Note: Summarized in Table 1.
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Summarized in Table 1.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: dorsal root ganglion |
Present |
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1D
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Expressed in both intracellular and surface membrane compartments in axons as well as cell bodies of neurons. The positive population also appeared to be reduced.
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Specimen
not shown:
embryonic day 12.0
(more )
Note: Summarized in Table 1.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Summarized in Table 1.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dorsal root ganglion |
Present |
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Expressed in both intracellular and surface membrane compartments in axons as well as cell bodies of neurons. Decreased number of positive neurons.
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Specimen
3B:
embryonic day 11.0
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: dorsal root ganglion |
Present |
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3B
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Positive cells had a clear neuronal morphology with the bipolar shape characteristic of young sensory neurons.
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3tm1Lfr
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Summarized in Table 2.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: dorsal root ganglion |
Present |
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5C
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Apoptosis of positive neurons were seen. But numerous positive neurons appeared morphologically normal. A loss of about 40% of positive neurons was seen in the mutant.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntrk3tm1Bbd/Ntrk3tm1Bbd
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Summarized in Table 2.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: dorsal root ganglion |
Present |
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6D
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In spite of the presence of numerous pyknotic figures, none were positive for Ntrk2/TrkB. Normal numbers of positive neurons were seen.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntrk3tm1Bbd/Ntrk3tm1Bbd
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntrk3tm1Bbd/Ntrk3tm1Bbd
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: dorsal root ganglion |
Present |
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The number of positive cells increased in the caudal-to-rostral direction.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3tm1Lfr
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dorsal root ganglion |
Present |
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Significant numbers of positive cells remained in the mutant.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3tm1Lfr
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: dorsal root ganglion |
Present |
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Significant numbers of positive cells remained in the mutant.
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