Dio3 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218428 Martinez ME, et al., Genomic imprinting variations in the mouse type 3 deiodinase gene between tissues and brain regions. Mol Endocrinol. 2014 Nov;28(11):1875-86
Assay type:	Northern blot
MGI Accession ID:	MGI:7735056
Gene symbol:	Dio3
Gene name:	deiodinase, iodothyronine type III

Probe:	Dio3 probe
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	4 kb (a)	2.7 kb (b)	2.2 kb (c)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Eye +/+	E15.5	TS23: eye	Not Applicable	Absent	Present	20 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Not Specified
Eye m-/p+	E15.5	TS23: eye	Present	Absent	Present	20 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Eye m+/p-	E15.5	TS23: eye	Present	Absent	Present	20 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Brain +/+	E15.5	TS23: brain	Not Applicable	Present	Present	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Not Specified
Brain m-/p+	E15.5	TS23: brain	Weak	Present	Present	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Brain m+/p-	E15.5	TS23: brain	Present	Present	Present	5 µg; poly-A+ RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Rest of Head +/+	E15.5	TS23: head	Not Applicable	Absent	Present	20 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6		Not Specified	Sample was the remaining tissue from the head after the eyes and brain were dissected.
Rest of Head m-/p+	E15.5	TS23: head	Weak	Absent	Present	20 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Sample was the remaining tissue from the head after the eyes and brain were dissected. Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Rest of Head m+/p-	E15.5	TS23: head	Present	Absent	Present	20 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Sample was the remaining tissue from the head after the eyes and brain were dissected. Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).

Notes:
(a) Mutated transcript. The abundance of the mutated 4-kb and the native 2.2-kb Dio3 transcript is similar in the eye of Dio3 m+/p- and Dio3 m-/p+ animals (lanes 2 and 3). In the nonneural head samples, the mutated transcript was much more abundant than the native transcript in Dio3 m+/p- mice, whereas the native Dio3 transcript was much more abundant than the mutated one in Dio3 m-/p+ mice (lanes 8 and 9). Similarly, in the fetal brain (lanes 5 & 6), transcripts that originated in the paternal allele are also more abundant, although the pattern is not as marked as in the tissues contained in the rest of the head.
(b) Brain specific wild type transcript.
(c) Wild type transcript.

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