Dio3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218428 Martinez ME, et al., Genomic imprinting variations in the mouse type 3 deiodinase gene between tissues and brain regions. Mol Endocrinol. 2014 Nov;28(11):1875-86
Assay type:	RT-PCR
MGI Accession ID:	MGI:7735055
Gene symbol:	Dio3
Gene name:	deiodinase, iodothyronine type III

Probe:	Dio3-pF, Dio3-pR mutated allele
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Gapdh.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Eye m-/p+	E15.5	TS23: eye	Present (a)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Eye m+/p-	E15.5	TS23: eye	Present (a)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Brain m-/p+	E15.5	TS23: brain	Present (b)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Brain m+/p-	E15.5	TS23: brain	Present (b)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).
Rest of Head m-/p+	E15.5	TS23: head	Present (c)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Sample was the remaining tissue from the head after the eyes and brain were dissected. Heterozygous mice that inherited the mutation from the mother (Dio3 m-/p+).
Rest of Head m+/p-	E15.5	TS23: head	Present (c)	1 µg; total RNA	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Dio3^tm1Stg/Dio3⁺	Not Specified	Sample was the remaining tissue from the head after the eyes and brain were dissected. Heterozygous mice that inherited the mutation from the father (Dio3 m+/p-).

Notes:
(a) Mutated Dio3 mRNA was expressed at similar levels in the eyes of Dio3 m-/p+ and Dio3 m+/p- fetuses.
(b) In the brain, the expression of the mutated Dio3 mRNA was reduced in Dio3 m+/p- fetuses compared with that in Dio3 m-/p+ fetuses.
(c) In the rest of the fetal head, reduction was more marked, indicating a stronger preferential Dio3 expression from the paternal allele.

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