Gene Expression Data
Assay Details
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Assay
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Reference:
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J:57046
Zhadanov AB, et al., Absence of the tight junctional protein AF-6 disrupts epithelial cell-cell junctions and cell polarity during mouse development. Curr Biol. 1999 Aug 26;9(16):880-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7734723
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Gene symbol:
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Tjp1
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Gene name:
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tight junction protein 1
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Antibody:
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anti-ZO-1
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Assay notes:
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Two Afdn antibodies were described, but the one used in this assay was not detailed. Anti-AF-6 MGI:7734709 may have been used in this assay.
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Results
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Specimen
6a:
embryonic day 7.5
(more  )
Note: Double labeled: green - Tjp1; red - Afdn (assay MGI:7734722).
Specimen
6a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Tjp1; red - Afdn (assay MGI:7734722).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
6a
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Expression overlapping with that of Afdn.
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Specimen
7a:
embryonic day 7.5
(more )
Specimen
7a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7b:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Afdntm1Mrcr/Afdntm1Mrcr
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
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7b
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Expression not altered compared to wild type.
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Analysis Tools
