Gene Expression Data
Assay Details
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Assay
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Reference:
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J:57046
Zhadanov AB, et al., Absence of the tight junctional protein AF-6 disrupts epithelial cell-cell junctions and cell polarity during mouse development. Curr Biol. 1999 Aug 26;9(16):880-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7734720
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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T probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3a +/+ (left):
embryonic day 7.5
(more  )
Specimen
3a +/+ (left):
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3a +/+ (right):
embryonic day 7.5
(more )
Specimen
3a +/+ (right):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3a -/- (left):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Afdntm1Mrcr/Afdntm1Mrcr
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
3a -/- (left)
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Expression detected, suggesting presence of primitive streak, despite abnormal morphology of mesoderm.
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Specimen
3a -/- (right):
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Afdntm1Mrcr/Afdntm1Mrcr
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
3a -/- (right)
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Expression detected, suggesting presence of primitive streak, despite abnormal morphology of mesoderm.
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Specimen
3b +/+ (left):
embryonic day 8.5
(more )
Specimen
3b +/+ (left):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3b +/+ (right):
embryonic day 8.5
(more )
Specimen
3b +/+ (right):
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3b -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Afdntm1Mrcr/Afdntm1Mrcr
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Present |
Regionally restricted |
3b -/-
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Expression confined to small patches of cells, possibly corresponding to axial mesoderm.
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Analysis Tools