Probe:	Cacna1d cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: lung	Present		4A E12.5
TS20: midbrain	Present		4A E12.5
TS20: neocortex	Present		4A E12.5
TS20: endocrine gland	Present	Regionally restricted	4A E12.5	Expressed in the adrenal gland.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		4A E14.5
TS22: spinal cord	Present		4A E14.5
TS22: tongue	Present		4A E14.5
TS22: stomach	Present	Regionally restricted	4A E14.5	Expressed in the stomach wall.

Structure	Level	Pattern	Image	Note
TS20: neural retina	Strong	Widespread	4B	Expressed in the whole neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Strong	Regionally restricted	4C	Expressed in the whole neuroblastic layer, but no staining was detected at the outermost cells.

Structure	Level	Pattern	Image	Note
TS24: retina layer	Present	Regionally restricted	4D	Expressed in the inner neuroblastic layer, with the highest signal intensity in the area around the developing lens.

Structure	Level	Pattern	Image	Note
TS26: retina ganglion cell layer	Present	Regionally restricted	4E	Expressed in a subset of the cells.
TS26: retina inner nuclear layer	Strong		4E
TS26: retina layer	Weak	Regionally restricted	4E	Low-intensity staining was present in the neuroepithelium surrounding the ganglion cells and the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Strong		4F
TS28: retina ganglion cell layer	Strong	Regionally restricted	4F	Expressed in a subset of the cells.
TS28: photoreceptor layer inner segment	Present		4F
TS28: retina outer nuclear layer	Present		4F

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Strong		4G
TS28: retina ganglion cell layer	Strong	Regionally restricted	4G	Expressed in a subset of the cells.
TS28: photoreceptor layer inner segment	Present		4G
TS28: retina outer nuclear layer	Present		4G

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Strong		4H
TS28: retina ganglion cell layer	Strong	Regionally restricted	4H	Expressed in a subset of the cells.
TS28: photoreceptor layer inner segment	Present		4H
TS28: retina outer nuclear layer	Present		4H

Structure	Level	Pattern	Image	Note
TS24: inner ear	Present		4I
TS24: cochlea	Present		4J , 4I
TS24: inner ear vestibular component	Present		4J , 4I

Structure	Cell Type	Level	Pattern	Image	Note
TS24: organ of Corti	cochlear inner hair cell	Present		4L

Structure	Cell Type	Level	Pattern	Image	Note
TS24: organ of Corti	cochlear inner hair cell	Present		4M
TS24: cochlear ganglion	spiral ganglion neuron	Present		4M

Structure	Cell Type	Level	Pattern	Image	Note
TS24: macula of utricle	macular hair cell	Present		4N

Structure	Level	Pattern	Image	Note
TS24: semicircular canal	Present	Regionally restricted	4O	Expressed in the crista ampullaris of the semicircular canals.

Structure	Level	Pattern	Image	Note
TS20: 4th ventricle choroid plexus	Present
TS20: duodenum	Present
TS20: heart	Present
TS20: inner ear	Present
TS20: jaw	Present
TS20: metanephros	Present
TS20: olfactory epithelium	Present
TS20: spinal cord	Present
TS20: thalamus	Present
TS20: tongue	Present
TS20: trigeminal ganglion	Present
TS20: umbilical cord	Present

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle choroid plexus	Present
TS22: adrenal gland	Present
TS22: duodenum	Present
TS22: heart	Present
TS22: inner ear	Present
TS22: jaw	Present
TS22: lung	Present
TS22: main olfactory bulb	Present
TS22: metanephros	Present
TS22: midbrain	Present
TS22: neocortex	Present
TS22: thalamus	Present
TS22: trigeminal ganglion	Present
TS22: umbilical cord	Present

Structure	Level	Pattern	Image	Note
TS24: 4th ventricle choroid plexus	Present
TS24: adrenal gland	Present
TS24: duodenum	Present
TS24: heart	Present
TS24: jaw	Present
TS24: lung	Present
TS24: main olfactory bulb	Present
TS24: metanephros	Present
TS24: midbrain	Present
TS24: neocortex	Present
TS24: olfactory epithelium	Present
TS24: spinal cord	Present
TS24: stomach wall	Present
TS24: thalamus	Present
TS24: tongue	Present
TS24: trigeminal ganglion	Present
TS24: umbilical cord	Present

Structure	Level	Pattern	Image	Note
TS26: lung	Present
TS26: metanephros	Present
TS26: olfactory epithelium	Present
TS26: spinal cord	Present
TS26: brain	Strong
TS26: inner ear	Strong