Assay
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Reference:
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J:164341
Kersten FF, et al., Association of whirlin with Cav1.3 (alpha1D) channels in photoreceptors, defining a novel member of the usher protein network. Invest Ophthalmol Vis Sci. 2010 May;51(5):2338-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7734684
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Gene symbol:
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Cacna1d
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Gene name:
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calcium channel, voltage-dependent, L type, alpha 1D subunit
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Antibody:
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anti-Cav1.3
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Detection system:
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Secondary antibody
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Results
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Specimen
3A:
postnatal
(more )
Note: Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6JOlaHsd
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Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal rod cell |
Present |
|
3A
|
Expressed in the collarlike extension of the apical inner segment, and in the base of the outer segment.
|
Specimen
3B:
postnatal
(more )
Note: Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6JOlaHsd
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal rod cell |
Present |
|
3B
|
Expressed in the collarlike extension of the apical inner segment, at the basal body complex, in the connecting cilium and in the base of the outer segment.
|
Specimen
3C:
postnatal
(more )
Note: Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6JOlaHsd
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal rod cell |
Present |
|
3C
|
Expressed at the basal body complex, in the connecting cilium, and in the base of the outer segment.
|
Specimen
3D:
postnatal
(more )
Note: Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6JOlaHsd
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal rod cell |
Present |
|
3D
|
Expressed in the connecting cilium.
|
Specimen
3E:
postnatal
(more )
Note: Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
Specimen
3E:
(close )
Genetic Background:
|
C57BL/6JOlaHsd
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal rod cell |
Present |
|
3E
|
Expressed at the synapses of the photoreceptor cells.
|
Specimen
3F:
postnatal
(more )
Note: Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
Specimen
3F:
(close )
Genetic Background:
|
C57BL/6JOlaHsd
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Vibratome sections were used. After staining, fixed with 0.5% OsO4, then embedded in araldite and ultrathin sections were analyzed with a transmission electron microscope.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: photoreceptor layer |
retinal rod cell |
Present |
|
3F
|
Expressed at the synapses of the photoreceptor cells.
|
|