Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56295
Ohshima T, et al., Migration defects of cdk5(-/-) neurons in the developing cerebellum is cell autonomous. J Neurosci. 1999 Jul 15;19(14):6017-26
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7733406
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Gene symbol:
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Calb1
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Gene name:
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calbindin 1
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Antibody:
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anti-calbindin D-28k
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Two antibodies were described MGI:7733400 MGI:7733399; the one used in this assay was not specified.
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Results
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Specimen
1A:
embryonic day 18.5; Cdk5tm1Kul/Cdk5 +
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129S4/SvJ * 129/SvJ * C57BL/6
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Mutant Allele(s):
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Cdk5tm1Kul/Cdk5+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1B:
(close )
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Genetic Background:
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involves: 129S4/SvJ * 129/SvJ * C57BL/6
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Mutant Allele(s):
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Cdk5tm1Kul/Cdk5tm1Kul
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: cerebellum |
Purkinje cell |
Present |
Regionally restricted |
1B
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In cdk5-/- mice, virtually no Purkinje cells are found in the normal cortical position. Instead, nearly all calbindin-positive cells are located deep in the cerebellar parenchyma.
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Analysis Tools
