Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74344
Nakashima K, et al., The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell. 2002 Jan 11;108(1):17-29
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7720200
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Gene symbol:
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Sp7
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Gene name:
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Sp7 transcription factor 7
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
2F upper:
embryonic day 14.5; Sp7tm1Crm/Sp7 +
(more  )
Specimen
2F upper:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/Sp7+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: embryo |
Present |
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2F upper
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Expression pattern reproduces endogenous expression, very similar in heterozygous and homozygous mutants
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Specimen
2F lower:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/Sp7tm1Crm
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: embryo |
Present |
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2F lower
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Expression pattern reproduces endogenous expression, very similar in heterozygous and homozygous mutants
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Specimen
E12.5 not shown:
embryonic day 12.5; Sp7tm1Crm/?
(more )
Specimen
E12.5 not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/?
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Specimen
E13.5 not shown:
embryonic day 13.5; Sp7tm1Crm/?
(more )
Specimen
E13.5 not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/?
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Specimen
3C1:
embryonic day 14.5; Sp7tm1Crm/Sp7 +
(more )
Note: Double labeled: blue - Sp7; brown - von Kossa staining.
Specimen
3C1:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/Sp7+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Sp7; brown - von Kossa staining.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: jaw bone |
Present |
Regionally restricted |
3C1
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Expression in condensed mesenchyme.
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Specimen
3C2:
embryonic day 14.5; Sp7tm1Crm/ Sp7tm1Crm
(more )
Note: Double labeled: blue - Sp7; brown - von Kossa staining.
Specimen
3C2:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/Sp7tm1Crm
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Sp7; brown - von Kossa staining.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: jaw bone |
Present |
Regionally restricted |
3C2
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Expression in condensed mesenchyme.
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Specimen
3C3:
embryonic day 14.5; Sp7tm1Crm/Sp7 +
(more )
Note: Double labeled: blue - Sp7; brown - von Kossa staining.
Specimen
3C3:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/Sp7+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Sp7; brown - von Kossa staining.
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Specimen
3C4:
embryonic day 14.5; Sp7tm1Crm/ Sp7tm1Crm
(more )
Note: Double labeled: blue - Sp7; brown - von Kossa staining.
Specimen
3C4:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/Sp7tm1Crm
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: blue - Sp7; brown - von Kossa staining.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: humerus cartilage condensation |
Present |
Regionally restricted |
3C4
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Exression in mesenchymal cells in periosteum/perichodrium and in wedge-shaped mesenchyme .
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Specimen
E17.5 not shown:
embryonic day 17.5; Sp7tm1Crm/?
(more )
Specimen
E17.5 not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sp7tm1Crm/?
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: humerus |
Present |
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Authors report that expression patterns were reproduced in heterozygous and homozygous mutants at E17.5
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| TS25: jaw bone |
Present |
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Authors report that expression patterns were reproduced in heterozygous and homozygous mutants at E17.5
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Analysis Tools