Gene Expression Data
Assay Details
Assay
|
Reference:
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J:170495
Ikeda K, et al., Six1 is indispensable for production of functional progenitor cells during olfactory epithelial development. Int J Dev Biol. 2010;54(10):1453-64
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7720113
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Gene symbol:
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Six1
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Gene name:
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sine oculis-related homeobox 1
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Antibody:
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anti-Six1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2Aa/c/e:
embryonic day 13.5
(more )
Note: Double labeled: green - Six1; red - Mki67 (assay MGI:7720114).
Specimen
2Aa/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Six1; red - Mki67 (assay MGI:7720114).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: olfactory epithelium |
Present |
|
2Aa
,
2Ac
,
2Ae
|
Coexpressed with Mki67 in the apical and basal layers.
|
Specimen
2Ba/c/e:
embryonic day 13.5
(more )
Note: Double labeled: green - Six1; red - Neurod1 (assay MGI:7720115).
Specimen
2Ba/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Six1; red - Neurod1 (assay MGI:7720115).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: olfactory epithelium |
Present |
Regionally restricted |
2Bc
,
2Ba
,
2Be
|
Expressed in the apical and basal layers. Coexpressed with Neurod1 in the basal layer cells.
|
Specimen
2Ca/c/e:
embryonic day 13.5
(more )
Note: Double labeled: green - Six1; red - Hes1 (assay MGI:7720116).
Specimen
2Ca/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Six1; red - Hes1 (assay MGI:7720116).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: olfactory epithelium |
Present |
Regionally restricted |
2Cc
,
2Ce
,
2Ca
|
Expressed in the apical and basal layers. Coexpressed with Hes1 in the apical cells.
|
TS21: nasal mesenchyme |
Moderate |
Regionally restricted |
2Ca
,
2Cc
,
2Ce
|
Moderate to low expression in the mesenchyme underlying the olfactory epithelium.
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Specimen
2Da/c/e:
embryonic day 16.5
(more )
Note: Double labeled: green - Six1; red - Neurod1 (assay MGI:7720115).
Specimen
2Da/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Six1; red - Neurod1 (assay MGI:7720115).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: olfactory epithelium |
Present |
Regionally restricted |
2Dc
,
2De
,
2Da
|
Expressed in the apical and basal layers. Coexpressed with Neurod1 in the basal layer cells.
|
Specimen
2Ea/c/e:
embryonic day 16.5
(more )
Note: Double labeled: green - Six1; red - Uchl1 (assay MGI:7720117).
Specimen
2Ea/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Six1; red - Uchl1 (assay MGI:7720117).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: olfactory epithelium |
Present |
Regionally restricted |
2Ea
,
2Ec
,
2Ee
|
Expressed in the apical and basal layers. Not coexpressed with Uchl1/PGP9.5.
|
Specimen
2Fa/c/e:
embryonic day 16.5
(more )
Note: Double labeled: green - Six1; red - Omp (assay MGI:7720118).
Specimen
2Fa/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Six1; red - Omp (assay MGI:7720118).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: olfactory epithelium |
Present |
Regionally restricted |
2Fc
,
2Fe
,
2Fa
|
Expressed in the apical and basal layers. Not coexpressed with Omp.
|
Specimen
2Ga/c/e:
postnatal month 1.5
(more )
Note: Double labeled: green - Six1; red - SUS-4.
Specimen
2Ga/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 1.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Six1; red - SUS-4.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: olfactory epithelium |
olfactory epithelial supporting cell |
Present |
|
2Ge
,
2Ga
,
2Gc
|
Expressed in the nuclei. Supporting cells/sustentacular cells were marked by SUS-4.
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Specimen
2Ha/c/e:
postnatal month 1.5
(more )
Note: Double labeled: green - Six1; red - Mki67 (assay MGI:7720114).
Specimen
2Ha/c/e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal month 1.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Six1; red - Mki67 (assay MGI:7720114).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: olfactory epithelium |
globose cell of olfactory epithelium |
Present |
|
2He
,
2Hc
,
2Ha
|
Coexpressed with Mki67.
|
TS28: olfactory epithelium |
olfactory epithelial supporting cell |
Present |
|
2He
,
2Hc
,
2Ha
|
Coexpressed with Mki67.
|
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